Crm at 20:15, 20 August 2018

2018-08-20T20:15:13Z

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Condition:1mM IPTG, 4oC,O/N

[[File:Protein QC 4 23 2018.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| Sample
| Name
| MW
| Note
|-
| M1
| SeeBlue
| 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 
| 
| 
|-
| 1
| 993(S)
| pCold-GST-M13-Gene8 
| 31.3
| y?
|-
| 2
| 992(S)
| pCold-GST-V5 
| 30.9
| y?
|-
| 3
| 991(S)
| pCold-GST-Flag 
| 30.5
| Y
|-
| 4
| 990(S)
| pCold-GST-HA 
| 30.6
| Y
|-
| 5
| 989(S)
| pCold-GST-Myc 
| 30.7
| Y
|-
| M2
| homemade protein standard
| 
| 
| 
|-
| 6
| 993(IB)
| 
| 
| 
|-
| 7
| 992(IB)
| 
| 
| 
|-
| 8
| 991(IB)
| 
| 
| 
|-
| 9
| 990(IB)
| 
| 
| 
|-
| 10
| 989(IB)
| 
| 
| 
|}

[[File:Protein QC 4 23 2018 (2).jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| Sample
| Name
| Type
| MW
| Note
|-
| M1
| seeblue
| 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 
| 
| 
| 
|-
| 1
| 947
| pT7A30-6His-pMBP 
| S
| 46.6
| Y
|-
| 2
| 986
| pT7A3-6His-NLS-Cas9-NLS-6xHis 
| S
| 164
| Y
|-
| 3
| 945
| pT7A30-pMBP 
| S
| 48.3
| Y
|-
| M2
| 
| 
| 
| 
| 
|-
| 4
| 947
| 
| IB
| 
| 
|-
| 5
| 986
| 
| IB
| 
| 
|-
| 6
| 945
| 
| IB
| 
| 
|}
[[Category:Protein Expression]]

Protein QC 4 23 18 - Revision history

Crm at 20:15, 20 August 2018