Crm at 19:14, 10 October 2018

2018-10-10T19:14:31Z

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Experiment condition:

15ml meduim, 0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.

'''Prptein Screening of 1000_942(+)_938_939_941'''

[[File:Img012.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| ID
| Name 
| Type
| MW
| Expression
| Note
| Pellet Type
|-
| M
| 
|
SeeBlue Protein

4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191

|  
| 
| 
| 
| 
|-
| 1
| 1000
| pT7A3-6His-NLS-FnCpf1-NLS-6xHis 
| unbroken cell pellet
| 157.1 
| Y
| 
| loose
|-
| 2
| 942(+)
| pT7A3-pMBP-VVN 
| unbroken cell pellet
| 24.8
|  ?
| 
| tight
|-
| 3
| 938
| pT7A30-PNGase F 
| unbroken cell pellet
| 40.1 
|  ?
| 
| loose
|-
| 4
| 939
| pET30b-Tte-ExoIII 
| unbroken cell pellet
| 31.3
| y
| 
| loose
|-
| 5
| 941
| pT7A3-pMBP-T7-Endo-1 
| unbroken cell pellet
| 63.4
| N
| 
| loose
|-
| 6
| 1000
| 
| S
| 
| Y
| 
| 
|-
| 7
| 942(+)
| 
| S
| 
|  ?
| 
| 
|-
| 8
| 938
| 
| S
| 
|  ?
| 
| 
|-
| 9
| 939
| 
| S
| 
| Y
| 
| 
|-
| 10
| 941
| 
| S
| 
|  ?
| 
| 
|-
| 11
| 941 in invitrogen Sample buffer
| 
| S
| 
| 
| 
| 
|}

'''Protein Screening of 3197_994_995_3225_3210'''

[[File:Img015.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| ID
| Name
| Type
| MW
| PI
| Expression
| Note
|-
| M
| 
|
SeeBlue Protein ladder

4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191

| 
| 
| 
| 
| 
|-
| 1
| 3197
| pT7A3-GST-BCMA 
| unbroken cell pellet
| 35 
| 
| 
| 
|-
| 2
| 994
| pT7A3-GST-CD133-EC2 
| unbroken cell pellet 
| 53 
| 
| 
| 
|-
| 3
| 995
| pT7A3-GST-CD133-EC3 
| unbroken cell pellet 
| 50.1 
| 
| 
| 
|-
| 4
| 3225
| pT7A3-GST-MICA 
| unbroken cell pellet 
| 42.5 
| 
| 
| 
|-
| 5
| 3210
| pT7A30-Sumo-Ov-ASP1-BCMA 
| unbroken cell pellet 
| 36.7 
| 
| 
| 
|-
| M
| SeeBlue Protein Ladder
| 
| 
| 
| 
| 
| 
|-
| 6
| 3197
| 
| S
| 
| 
| Y
| 
|-
| 7
| 994
| 
| S
| 
| 
| Y
| 
|-
| 8
| 995
| 
| S
| 
| 
| ?
| 
|-
| 9
| 3225
| 
| S
| 
| 
| Y
| 
|-
| 10
| 3210
| 
| S
| 
| 
| Y
| 
|}

{| id="dataTable" align="center" class="list" cellspacing="1px" style="font-family: Arial, Verdana, sans-serif; font-size: 12px; color: black; border: 0px; background-color: rgb(224, 224, 239);"
|- style="background-color: rgb(251, 251, 239);"
| style="word-wrap: break-word; display: block; width: 600px;" | pT7A3-GST-BCMA
|}
[[Category:QC]] [[Category:Protein Expression]]

Protein QC 8 10 2018 - Revision history

Crm at 19:14, 10 October 2018