Crm at 19:24, 10 October 2018

2018-10-10T19:24:42Z

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Experiment condition:

M1:Seeblue protein ladder

M2:GeneScript Multi prestained protein ladder

M3:Spectra BR Multicolor protein ladder

'''Protein expression Screening 3197_994_995_3225_3210'''

15ml medium, 0.5mM IPTG induce at RT for over night (around 14 hours), freeze and thraw on dry ice three times.

[[File:Img016.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width: 521px;"
|-
| Lane
| #
| Name
| Type
| MW
| Note
| Pellet Type
|-
| M1
| SeeBlue protein  ladder
| 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 
| 
| 
| 
| 
|-
| 1
| 3197
| pT7A3-GST-BCMA 
| unbroken cell pellet supertant 
| 35
| Y
| loose
|-
| 2
| 994
| pT7A3-GST-CD133-EC2 
| unbroken cell pellet supertant 
| 53
| Y
| loose
|-
| 3
| 995
| pT7A3-GST-CD133-EC3 
| unbroken cell pellet supertant 
| 50.1
| Y
| tight
|-
| 4
| 3225
| pT7A3-GST-MICA 
| unbroken cell pellet supertant 
| 42.5
| Y
| tight
|-
| 5
| 3210
| pT7A30-Sumo-Ov-ASP1-BCMA 
| unbroken cell pellet supertant 
| 36.7
|  ?
| loose
|-
| M2
|
GeneScript

Broad Multi Color Pre-Stained Protein Standard

| 5kD, 15kD, 30kD(red), 35kD, 50kD(Green), 65kD, 95kD,130kD,175kD, and 270kD(purple). 
| 
| 
| 
| 
|-
| 6
| 3197
| 
| S
| 
| Y
| 
|-
| 7
| 994
| 
| S
| 
| Y
| 
|-
| 8
| 995
| 
| S
| 
| Y
| 
|-
| 9
| 3225
| 
| S
| 
| Y
| 
|-
| 10
| 3210
| 
| S
| 
| Y
| 
|}

'''Protein expression Screening 3218_824_999_944'''

15ml medium, 0.5mM IPTG induce at RT for over night (around 14 hours), freeze and thraw on dry ice three times.

[[File:Img017.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| #
| Name
| Type
| MW
| Note
| Pellet Type
|-
| M1
| SeeBlue
| 4, 6, 14, 17(red), 28, 38, 49, 62, 97(yellow), 191 
| 
| 
| 
| 
|-
| 1
| 3218
| pT7A30-ULP1 
| unbroken cell pellet
| 
|  ?
| loose
|-
| 2
| 3191
| pT7A3-GST-Avitag 
| unbroken cell pellet 
| 31
| Y
| loose
|-
| 3
| 824
| pET28b-VVTopo 
| unbroken cell pellet 
| 39.9
| Y
| loose
|-
| 4
| 999
| pT7A30-SssI 
| unbroken cell pellet 
| 46
| N
| tight
|-
| 5
| 944
| <div><div>pET30b-pfu-exo-</div></div><div>
{| id="dataTable" align="center" class="list" cellspacing="1px" style="font-family: Arial, Verdana, sans-serif; font-size: 12px; color: black; border: 0px; background-color: rgb(224, 224, 239);"
|-
|
|}
</div>
| unbroken cell pellet 
| 91.1
| N
| loose
|-
| M3
| Spectra BR protein ladder
| 5,10,25,35,40(red),50(green),70(brown),100,140(purple),260
| 
| 
| 
| 
|-
| 6
| 3218
| 
| S
| 
| 
| 
|-
| 7
| 3191
| 
| S
| 
| Y
| 
|-
| 8
| 824
| 
| S
| 
| Y
| 
|-
| 9
| 999
| 
| S
| 
| N
| 
|-
| 10
| 944
| 
| S
| 
| Y
| 
|}

Protein QC 8 14 2018 - Revision history

Crm at 19:24, 10 October 2018