https://eln.excellgen.com/index.php?action=history&feed=atom&title=Purification_of_Taq_DNA_Polymerase Purification of Taq DNA Polymerase - Revision history 2026-10-19T14:17:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Purification_of_Taq_DNA_Polymerase&diff=15544&oldid=prev Paul.xu: Created page with "== Buffers == '''Lysis Buffer''' {| border="1" cellspacing="1" cellpadding="1" style="width:500px;" |- | 20 mM Tris-HCl, pH 8.0<br/> | <br/> |- | 500 mM NaCl<br/> | <br/> |-..." 2017-01-20T23:05:24Z <p>Created page with &quot;== Buffers == &#039;&#039;&#039;Lysis Buffer&#039;&#039;&#039; {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot; |- | 20 mM Tris-HCl, pH 8.0&lt;br/&gt; | &lt;br/&gt; |- | 500 mM NaCl&lt;br/&gt; | &lt;br/&gt; |-...&quot;</p> <p><b>New page</b></p><div>== Buffers ==<br /> <br /> &#039;&#039;&#039;Lysis Buffer&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 500 mM NaCl&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 0.5% NP 40<br /> | &lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | H2O<br /> | to 1 L<br /> |}<br /> <br /> &#039;&#039;&#039;Ni Washing Buffer 1&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0<br /> | &lt;br/&gt;<br /> |-<br /> | 1 M NaCl<br /> | &lt;br/&gt;<br /> |-<br /> | 0.5% NP40<br /> | &lt;br/&gt;<br /> |-<br /> | 10 mM Imadazole<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Ni Washing Buffer 2&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 1 M NaCl<br /> | &lt;br/&gt;<br /> |-<br /> | 0.5% NP40<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Ni Elution Buffer 1&#039;&#039;&#039;<br /> [[Category:Protocols]]<br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM &amp;nbsp;Tris-Hcl, pH 8.0<br /> | &lt;br/&gt;<br /> |-<br /> | 50 mM NaCl<br /> | &lt;br/&gt;<br /> |-<br /> | 150 mM Imadazole<br /> | &lt;br/&gt;<br /> |-<br /> | 5% Glycerol<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Ni Elution Buffer 2&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0<br /> | &lt;br/&gt;<br /> |-<br /> | 50 mM NaCl<br /> | &lt;br/&gt;<br /> |-<br /> | 10 mM EDTA<br /> | &lt;br/&gt;<br /> |-<br /> | 5% Glycerol<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Q HP Buffer 1&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0<br /> | &lt;br/&gt;<br /> |-<br /> | 50 mM KCl<br /> | &lt;br/&gt;<br /> |-<br /> | 5% Trehalose<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Q HP Buffer 2&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | 20 mM Tris-HCl, pH 8.0&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 500 mM KCl&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 5% Trehalose&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}</div> Paul.xu