https://eln.excellgen.com/index.php?action=history&feed=atom&title=QPCR QPCR - Revision history 2026-10-18T06:09:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=QPCR&diff=3968&oldid=prev Paul.xu: Created page with "10x Platinum taq buffer (Invitrogen)&nbsp; 2.5μl&nbsp; <br>50mM MgCl 2 (→ 1.5mM)&nbsp; 0.75μl <br>template DNA&nbsp; 2.5μl <br>10μM DLP (→ 250nM)&nbsp; 0.625μl <br>50x R..." 2012-04-23T02:36:26Z <p>Created page with &quot;10x Platinum taq buffer (Invitrogen)  2.5μl  &lt;br&gt;50mM MgCl 2 (→ 1.5mM)  0.75μl &lt;br&gt;template DNA  2.5μl &lt;br&gt;10μM DLP (→ 250nM)  0.625μl &lt;br&gt;50x R...&quot;</p> <p><b>New page</b></p><div>10x Platinum taq buffer (Invitrogen)&amp;nbsp; 2.5μl&amp;nbsp; &lt;br&gt;50mM MgCl 2 (→ 1.5mM)&amp;nbsp; 0.75μl &lt;br&gt;template DNA&amp;nbsp; 2.5μl &lt;br&gt;10μM DLP (→ 250nM)&amp;nbsp; 0.625μl &lt;br&gt;50x Rox (Invitrogen; → 1x)&amp;nbsp; 0.5μl &lt;br&gt;c_qPCR_v2.1 (→ 300nM)&amp;nbsp; 0.75μl &lt;br&gt;PE_qPCR_v2.2 (→ 300nM)&amp;nbsp; 0.75μl &lt;br&gt;2.5mM dNTPs (→ 200μM)&amp;nbsp; 2μl &lt;br&gt;Platinum Taq (Invitrogen; → 0.04 units / μl)&amp;nbsp; 0.1μl &lt;br&gt;H2O&amp;nbsp; 14.525μl &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> 94C for 2 min&lt;br&gt;94C 15sec&lt;br&gt;62C 15sec&lt;br&gt;72C 32sec&lt;br&gt;x 40&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;&lt;br&gt;<br /> <br /> [[Category:Protocols]]</div> Paul.xu