https://eln.excellgen.com/index.php?action=history&feed=atom&title=Rabbit_Antibody_CloningRabbit Antibody Cloning - Revision history2026-10-18T04:23:26ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Rabbit_Antibody_Cloning&diff=18978&oldid=prevPaul.xu at 18:57, 10 August 20182018-08-10T18:57:47Z<p></p>
<p><b>New page</b></p><div>== Signal Peptide ==<br />
<pre>atggagactgggctgcgctggcttctcctggtcgctgtgctcaaaggtgtccagtgt<br />
M E T G L R W L L L V A V L K G V Q C </pre><br />
<br />
== VH ==<br />
<pre>cagtccctggaggagtccgggggtcgcctggtcaagcctggaggaaccctgacactcacctgc<br />
Q S L E E S G G R L V K P G G T L T L T C <br />
acagtctctggaatcgacctcagtagctcgggagtgagctgggtccgccaggctccaggg<br />
T V S G I D L S S S G V S W V R Q A P G <br />
aaggggctggaatacatcggatacattagtagcagtggtagcacatactacgcgagctgg<br />
K G L E Y I G Y I S S S G S T Y Y A S W <br />
gcgaaaggccgattcaccatctccaaaacctcgtcgaccacggtggagctgaaagtgacc<br />
A K G R F T I S K T S S T T V E L K V T <br />
agtctgacaactgaggacacggccacctatttctgtagtagaaatggtctaggggccttg<br />
S L T T E D T A T Y F C S R N G L G A L <br />
tggggccaaggcaccctggtcaccgtt<br />
W G Q G T L V T V <br />
</pre><br />
<br />
== IgG Constant Heavy (allele 3/2) ==<br />
<pre>tcgagtgggcaacctaaggctccatcag<br />
S S G Q P K A P S ....</pre><br />
<br />
TCGAGTGGGCAACCTAAGGCTCCATCAGTCTTCCCACTGGCCCCCTGCTGCGGGGACACACCCAGCTCCACGGTGACCCTGGGCTGCCTGGTCAAAGGCTACCTCCCGGAGCCAGTGACCGTGACCTGGAACTCGGGCACCCTCACCAATGGGGTACGCACCTTCCCGTCCGTCCGGCAGTCCTCAGGCCTCTACTCGCTGAGCAGCGTGGTGAGCGTGACCTCAAGCAGCCAGCCCGTCACCTGCAACGTGGCCCACCCAGCCACCAACACCAAAGTGGACAAGACCGTTGCG CCCTCGACATGCAGCAAGCCCACGTGCCCACCCCCTGAACTCCTGGGGGGACCGTCTGTCTTCATCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCACGCACCCCCGAGGTCACATGCGTGGTGGTGGACGTGAGCCAGGATGACCCCGAGGTGCAGTTCACATGGTACATAAACAACGAGCAGGTGCGCACCGCCCGGCCGCCGCTACGGGAGCAGCAGTTCAACAGCACGATCCGCGTGGTCAGCACCCTCCCCATCGCGCACCAGGACTGGCTGAGGGGCAAGGAGTTCAAGTGCAAAGTCCACAACAAGGCACTCCCGGCCCCCATCGAGAAAACCATCTCCAAAGCCAGAGGGCAGCCCCTGGAGCCGAAGGTCTACACCATGGGCCCTCCCCGGGAGGAGCTGAGCAGCAGGTCGGTCAGCCTGACCTGCATGATCAACGGCTTCTACCCTTCCGACATCTCGGTGGAGTGGGAGAAGAACGGGAAGGCAGAGGACAACTACAAGACCACGCCGGCCGTGCTGGACAGCGACGGCTCCTACTTCCTCTACAGCAAGCTCTCAGTGCCCACGAGTGAGTGGCAGCGGGGCGACGTCTTCACCTGCTCCGTGATGCACGAGGCCTTGCACAACCACTACACGCAGAAGTCCATCTCCCGCTCTCCGGGTAAATGAGCGCTCCTAGGTCTAGACCTAG<br />
<br />
SSGQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTCSKPTCPPPELLGGPSVFIFPPKPKDTLMISRTPEVTCVVVDVSQDDPEVQFTWYINNEQVRTARPPLREQQFNSTIRVVSTLPIAHQDWLRGKEFKCKVHNKALPAPIEKTISKARGQPLEPKVYTMGPPREELSSRSVSLTCMINGFYPSDISVEWEKNGKAEDNYKTTPAVLDSDGSYFLYSKLSVPTSEWQRGDVFTCSVMHEALHNHYTQKSISRSPGK<br />
<br />
Full length<br />
<pre>atgagctgggtccgacaggctccagggaaggagctggagtggatcggatacattagttat<br />
M S W V R Q A P G K E L E W I G Y I S Y <br />
ggtggtagtgcatactacgcgagctgggcgaaaagccgatccaccatcaccagaaacacc<br />
G G S A Y Y A S W A K S R S T I T R N T <br />
aacgagaacacggtgactctgaaaatgaccagtctgacagccgcggacacggccacctat<br />
N E N T V T L K M T S L T A A D T A T Y <br />
ttctgtgcgagacattggggcatctggggcccaggcaccctggtcaccgtc<br />
F C A R H W G I W G P G T L V T V </pre><br />
<br />
<br />
<pre>tcctcagggcaacctaaggctccatcagtcttcccactggccccctgctgcggggacaca<br />
S S G Q P K A P S V F P L A P C C G D T <br />
cccagctccacggtgaccctgggctgcctggtcaaaggctacctcccggagccagtgacc<br />
P S S T V T L G C L V K G Y L P E P V T <br />
gtgacctggaactcgggcaccctcaccaatggggtacgcaccttcccgtccgtccggcag<br />
V T W N S G T L T N G V R T F P S V R Q <br />
tcctcaggcctctactcgctgagcagcgtggtgagcgtgacctcaagcagccagcccgtc<br />
S S G L Y S L S S V V S V T S S S Q P V <br />
acctgcaacgtggcccacccagccaccaacaccaaagtggacaagaccgttgcaccctcg<br />
T C N V A H P A T N T K V D K T V A P S <br />
acatgcagcaagcccacgtgcccaccccctgaactcctggggggaccgtctgtcttcatc<br />
T C S K P T C P P P E L L G G P S V F I <br />
ttccccccaaaacccaaggacaccctcatgatctcacgcacccccgaggtcacatgcgtg<br />
F P P K P K D T L M I S R T P E V T C V <br />
gtggtggacgtgagccaggatgaccccgaggtgcagttcacatggtacataaacaacgag<br />
V V D V S Q D D P E V Q F T W Y I N N E <br />
caggtgcgcaccgcccggccgccgctacgggagcagcagttcaacagcacgatccgcgtg<br />
Q V R T A R P P L R E Q Q F N S T I R V <br />
gtcagcaccctccccatcacgcaccaggactggctgaggggcaaggagttcaagtgcaaa<br />
V S T L P I T H Q D W L R G K E F K C K <br />
gtccacaacaaggcactcccggcccccatcgagaaaaccatctccaaagccagagggcag<br />
V H N K A L P A P I E K T I S K A R G Q <br />
cccctggagccgaaggtctacaccatgggccctccccgggaggagctgagcagcaggtcg<br />
P L E P K V Y T M G P P R E E L S S R S <br />
gtcagcctgacctgcatgatcaacggcttctacccttccgacatctcggtggagtgggag<br />
V S L T C M I N G F Y P S D I S V E W E <br />
aagaacgggaaggcagaggacaactacaagaccacgccggccgtgctggacagcgacggc<br />
K N G K A E D N Y K T T P A V L D S D G <br />
tcctacttcctctacaacaagctctcagtgcccacgagtgagtggcagcggggcgacgtc<br />
S Y F L Y N K L S V P T S E W Q R G D V <br />
ttcacctgctccgtgatgcacgaggccttgcacaaccactacacgcagaagtccatctcc<br />
F T C S V M H E A L H N H Y T Q K S I S <br />
cgctctccgggtaaatga<br />
R S P G K - <br />
<pre><br />
<br />
<br />
== Light Chain Kappa1 ==<br />
<br />
GATCCAGTTGCACCTACTGTCCTCATCTTCCCACCAGCTGCTGATCAGGTGGCAACTGGAACAGTCACCATCGTGTGTGTGGCGAATAAATACTTTCCCGATGTCACCGTCACCTGGGAGGTGGATGGCACCACCCAAACAACTGGCATCGAGAACAGTAAAACACCGCAGAATTCTGCAGATTGTACCTACAACCTCAGCAGCACTCTGACACTGACCAGCACACAGTACAACAGCCACAAAGAGTACACCTGCAAGGTGACCCAGGGCACGACCTCAGTCGTCCAGAGCTTCAACAGGGGTGACTGCTAG<br />
<br />
DPVAPTVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGDC-<br />
<br />
<pre><br />
Aligment of some VL domains<br />
5 mdtraptqllgllllwlpgatfavvltqtpssvsaavggsvtiscqssqslynnnrlawy<br />
4 ----------------------alvmtqtpssvsaavggtvtincqasqsvgnnnnlvwy<br />
6 ----------------------dvvmtqtpspvsaavggtvsincqasqsvyknnylswy<br />
8 ---------------------mdvvmtqtpspvsaavgstvtincqasqsvynnhylswy<br />
1 ----------------------divmtqtpasvsaavggtvtincqasetis--nylawy<br />
2 ----------------lpgarcaydmtqtpasvevavggtvtikcqasqsis--tylswy<br />
7 ---------------------aevvmtqapssveaavggtvtikcqasrsin--nylswy<br />
9 mdmraptqllgllllwlpgarcdvvmtqtpasveapvggtvtikcqasqsvs--gycswy<br />
3 ---------------------mdvvmtqtpasveaavggtvtikcqasqniy--snlawy<br />
10 ----------------------dvvmtqtpasvsepvggtvtikcqasqsis--sylawy<br />
&nbsp;:**:*: *. **.:*:*.**:*..: **<br />
<br />
5 qqkagqppklliykastlesgvpsrfkgsgagtqftltisdvvcddaatyycag-yksn-<br />
4 qqkpgqppklliyrasnletgvpsrfkgsgsgteftltisdlecddaatyycagaysg--<br />
6 qqkpgqrpklliygasnlesgvpsrfkgsgsgteftltisdvqcddaatyyclgeygsr-<br />
8 qqkpgqppkrliyaastlesgvssrfkgsgsgtqftltisdvqcddaatyyclgsydcs-<br />
1 qqkpgqppklliykastlasgvssrfkgsgsgteytltisgvqcddaatyycqqgysisd<br />
2 qqkpgqrpklliyrastlasgvssrfkgsgsgteftltisgvecadaatyycqqgwsssn<br />
7 rqkpgqppklliyeasklasgvpsrfsgsgsgteftltisdlecadaatyycqglyfvsh<br />
9 qqkpgqppklliyrastlesgvpsrfsgsgsgtdftltisdlecadaatyycqsnynsgs<br />
3 qqkpgqrpklliykastlasgvssrfkgsgsgteftltisdlecadaatyycqctwydn-<br />
10 qqkpgqrprlliyetstlasgvpsrfkgsgsgtdftltisdlecadaatyycqstyenp-<br />
&nbsp;:** ** *: ***&nbsp;:*.*&nbsp;:** ***.***:**::*****.: * ******* &nbsp;: <br />
<br />
5 sddgtafgggtevvvkgdPVAPT<br />
4 --nvihfgggtevvvkgdPVAPT<br />
6 sd--ngfgggtevvvkgdPVAPT<br />
8 sadcfafgggtevvvkgdPVAPT<br />
1 --idnsfgggtevvvkgdPVAPT<br />
2 --venvfgggtevvvkgdPVAPT<br />
7 ssygsafgggtevvvkgdPVAPT<br />
9 sssaaafgggtevvvkgdPVAPT<br />
3 --tyvafgggtevvvkgdPVAPT<br />
10 --tyvsfgggtevgvkgdPVAPT<br />
******* *********<br />
</pre><br />
<br />
<br />
<br />
== Light Chain Kappa2 ==<br />
<br />
GATCCAGTTGCGCCTTCTGTCCTCCTCTTCCCACCATCTAAGGAGGAGCTGACAACTGGAACAGCCACCATCGTGTGCGTGGCGAATAAATTCTATCCCAGTGACATCACCGTCACCTGGAAGGTGGATGGCACCACCCAACAGAGCGGCATCGAGAACAGTAAAACACCGCAGAGCCCCGAAGACAATACCTACAGCCTGAGCAGCACTCTGTCACTGACCAGCGCACAGTACAACAGCCACAGCGTGTACACCTGCGAGGTGGTCCAAGGCTCAGCCTCACCGATCGTCCAGAGCTTCAATAGGGGTGACTGCTAG<br />
<br />
DPVAPSVLLFPPSKEELTTGTATIVCVANKFYPSDITVTWKVDGTTQQSGIENSKTPQSPEDNTYSLSSTLSLTSAQYNSHSVYTCEVVQGSASPIVQSFNRGDC&lt;/pre&gt;<br />
[[Category:Antibodies]]</div>Paul.xu