Paul.xu: Created page with "20x RapidRun Agarose Buffer 1.78 M Tris 0.57 M Taurine *0.01M EDTA adjust pH to 8.9, filter. Reagent Supplier purity Cat. no. approx. price
Taurine (125.15 g/mol) Sig..."

2012-03-26T22:45:43Z

Created page with "20x RapidRun Agarose Buffer *1.78 M Tris *0.57 M Taurine *0.01M EDTA adjust pH to 8.9, filter. Reagent Supplier purity Cat. no. approx. price Taurine (125.15 g/mol) Sig..."

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20x RapidRun Agarose Buffer

*1.78 M Tris

*0.57 M Taurine

*0.01M EDTA

adjust pH to 8.9, filter.

Reagent Supplier purity Cat. no. approx. price Taurine (125.15 g/mol) Sigma ≥99%: T0625-500G USD162.00 Tris base (121.14 g/mol) Sigma ≥99%: T1378-5KG USD291.00 EDTA (292.24 g/mol) SysBio kitchen ≥99%: NA NA

For 5 L of 20x buffer combine: 1113.8 g of Tris 345.25 g of Taurine 14.6 g (or 50 mL of 1M EDTA)

bring to 4.5 L with H2O. Adjust pH to 8.9 and filter. 

[[Category:Buffer_Recipe]]

Rapid Run Agarose Gel Buffer - Revision history

Paul.xu: Created page with "20x RapidRun Agarose Buffer *1.78 M Tris *0.57 M Taurine *0.01M EDTA adjust pH to 8.9, filter. Reagent Supplier purity Cat. no. approx. priceTaurine (125.15 g/mol) Sig..."

Paul.xu: Created page with "20x RapidRun Agarose Buffer 1.78 M Tris 0.57 M Taurine *0.01M EDTA adjust pH to 8.9, filter. Reagent Supplier purity Cat. no. approx. price
Taurine (125.15 g/mol) Sig..."