https://eln.excellgen.com/index.php?action=history&feed=atom&title=Real_Time_PCR_Buffer_for_Blood Real Time PCR Buffer for Blood - Revision history 2026-10-18T04:02:54Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Real_Time_PCR_Buffer_for_Blood&diff=15154&oldid=prev Paul.xu: Created page with "&nbsp;&nbsp; &nbsp;Concentration &nbsp;at 2.0X<br/>Component&nbsp;&nbsp; &nbsp;<br/>1 M Tris-HCl pH 8.0&nbsp;&nbsp; &nbsp;100 mM<br/>5U/μl AmpliTaq Gold (or other Taq&nbsp;&n..." 2016-07-31T01:43:14Z <p>Created page with &quot;    Concentration  at 2.0X&lt;br/&gt;Component    &lt;br/&gt;1 M Tris-HCl pH 8.0    100 mM&lt;br/&gt;5U/μl AmpliTaq Gold (or other Taq &amp;n...&quot;</p> <p><b>New page</b></p><div>&amp;nbsp;&amp;nbsp; &amp;nbsp;Concentration &amp;nbsp;at 2.0X&lt;br/&gt;Component&amp;nbsp;&amp;nbsp; &amp;nbsp;&lt;br/&gt;1 M Tris-HCl pH 8.0&amp;nbsp;&amp;nbsp; &amp;nbsp;100 mM&lt;br/&gt;5U/μl AmpliTaq Gold (or other Taq&amp;nbsp;&amp;nbsp; &amp;nbsp;0.5U/μl&lt;br/&gt;25 mM each dNTP&amp;nbsp;&amp;nbsp; &amp;nbsp;0.4 mM&lt;br/&gt;100 mM dUTP&amp;nbsp;&amp;nbsp; &amp;nbsp;0.8 mM&lt;br/&gt;2 M KCl&amp;nbsp;&amp;nbsp; &amp;nbsp;100 mM&lt;br/&gt;1 M MgCl2&amp;nbsp;&amp;nbsp; &amp;nbsp;8 mM&lt;br/&gt;3 μM ROX Pass. Ref.&amp;nbsp;&amp;nbsp; &amp;nbsp;70 nM&lt;br/&gt;20 mg/ml BSA (Roche)&amp;nbsp;&amp;nbsp; &amp;nbsp;0.08 mg/ml&lt;br/&gt;10% NaN3&amp;nbsp;&amp;nbsp; &amp;nbsp;0.02%&lt;br/&gt;50% Glycerol (or other polyhydroxy alcohol&amp;nbsp;&amp;nbsp; &amp;nbsp;16.00%&lt;br/&gt;H2O&amp;nbsp;&amp;nbsp; &amp;nbsp;varying amount<br /> <br /> <br /> [[Category:QPCR RT-PCR]]</div> Paul.xu