https://eln.excellgen.com/index.php?action=history&feed=atom&title=Regenerated_amorphous_cellulose 2026-10-19T19:18:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Regenerated_amorphous_cellulose&diff=11033&oldid=prev 2014-01-19T19:36:07Z

<p>Created page with &quot;== Synthesis == Microcrystalline cellulose – Avicel PH105 (20μm) is from FMC (Philadelphia,PA), &lt;br&gt;or SigmaCell Sigmacell Cellulose Type 20, 20 μm Add ~ 0.2g of microcrys...&quot;</p> <p><b>New page</b></p><div>== Synthesis ==<br /> <br /> Microcrystalline cellulose – Avicel PH105 (20μm) is from FMC (Philadelphia,PA), &lt;br&gt;or SigmaCell Sigmacell Cellulose Type 20, 20 μm <br /> <br /> Add ~ 0.2g of microcrystalline cellulose to 0.6 mL distilled water in a 50-mL centrifuge tube.&lt;br&gt;Add 10 ul of ice-cold 86% H3PO4 (i.e., commercial 85% grade) slowly into the slurry with vigorous stirring so that the final phosphoric acid concentration is ~ 83.2%.&lt;br&gt;The cellulose mixture will become transparent within a few minutes. &lt;br&gt;Incubate on ice for ~ 1 hour with occasional stirring. &lt;br&gt;Add ~ 40mL of ice-cold water while mixing/stiring, this will result in a white cloudy precipitate. &lt;br&gt;Spin 10,000×g at 4◦C for 20 min.&lt;br&gt;Suspend the pellet in ice-cold water.&lt;br&gt;Spin again to remove the phosphoric acid.&lt;br&gt;Repeat wash for 4 times.&lt;br&gt;Add 0.5mL of 2M Na2CO3 and 40mL of ice-cold distilled water to neutralize and suspend the cellulose pellet. &lt;br&gt;Spin, wash the pellet with distilled waters twice or until pH 5–7. <br /> <br /> Store the slurry as a 10g RAC/1L suspension solution at 4◦C in the presence of 0.2% sodium azide &lt;br&gt;&lt;br&gt;<br /> <br /> [[Category:Protocols]]</div>

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