Paul.xu: Created page with "I used to use streptomycin to eliminate nucleic acids from my proteins extracts. I usually precipitate the crude extract with streptomaycin at 4 C, then I centrifuge at 12,000..."

2017-05-03T21:24:27Z

Created page with "I used to use streptomycin to eliminate nucleic acids from my proteins extracts. I usually precipitate the crude extract with streptomaycin at 4 C, then I centrifuge at 12,000..."

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I used to use streptomycin to eliminate nucleic acids from my proteins extracts. I usually precipitate the crude extract with streptomaycin at 4 C, then I centrifuge at 12,000 rpm. Then using chromatrography you can elimininate the streptomycin or just with a desalting column,

<br/>use heparin-Sepharose affinity chromatography
[[Category:Protein Purification]]

Remove nuclease - Revision history

Paul.xu: Created page with "I used to use streptomycin to eliminate nucleic acids from my proteins extracts. I usually precipitate the crude extract with streptomaycin at 4 C, then I centrifuge at 12,000..."