Paul.xu: Created page with "'''Digestion''' Use 5 ul miniprep for digestion 1. make mix, per reaction 1.5 ul FastDigestion Buffer 0.3 ul enzyme 1 0.3 ul enzyme 2 (if required) 5 ul miniprep..."

2017-06-12T20:04:19Z

Created page with "'''Digestion''' Use 5 ul miniprep for digestion 1. make mix, per reaction 1.5 ul FastDigestion Buffer 0.3 ul enzyme 1 0.3 ul enzyme 2 (if required) 5 ul miniprep..."

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'''Digestion'''

Use 5 ul miniprep for  digestion

1. make mix, per reaction

1.5 ul FastDigestion Buffer

0.3 ul enzyme 1

0.3 ul enzyme 2 (if required)

5 ul miniprep

H2O to 15 ul

2. Incubate at 37 oC  for 30 min in an incubator 

3. Add 5 ul 6X GRGreen loading buffer

4. Run on 1% agarose gel

5. Take a picture using blue light transilluminator 

6. Process gel picture and upload to ELN

7. Document all experimental details
[[Category:Protocols]]

Restriction Digestion - Revision history

Paul.xu: Created page with "'''Digestion''' Use 5 ul miniprep for digestion 1. make mix, per reaction 1.5 ul FastDigestion Buffer 0.3 ul enzyme 1 0.3 ul enzyme 2 (if required) 5 ul miniprep..."