Paul.xu at 12:11, 22 June 2017

2017-06-22T12:11:57Z

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== Hia5 ==

'''Not protected:''' BglI (one) Nhe1 Sma1 '''Unknown''' Not1 Eag1 Nco1 Bamh1 Kpn1 Sal1

 '''Summary of the sensitivity of different REases to DNA modifications introduced by the Hia5 enzyme'''
<div>
{|
|-
!
REase

!
Recognition site

!
Number of sites in pHia5ET

!
Sensitivity to m6A

!
Cleavage

|-
| BglII
| '''A'''GaTCT
| 1
| S
| N
|-
| Bsh1285I
| CGRYCG R'''>A'''/G
| 5
| S
| N
|-
| Bsu15I
| '''A'''TCG'''A'''T
| 2
| S
| N
|-
| CviQI
| GT'''A'''C
| 3
| S
| N
|-
| DraI
| TTT'''AAA'''
| 2
| S
| N
|-
| Eco32I/Eco32I
| G'''A'''T'''A'''TC
| 0 (1)b
| S
| N
|-
| EcoRI
| G'''AA'''TTC
| 0 (1)b
| S
| N
|-
| HincII
| GTYR'''A'''C
| 1 (GTTAAC)
| S
| N
|-
| HindIII
| '''A'''aGCTT
| 0 (1)b
| S
| N
|-
| HinfI
| G'''A'''NTC
| 19
| S
| N
|-
| HphI
| GGTG'''A'''/TCACC
| 16
| S
| N
|-
| KspAI
| GTTA'''A'''C
| 1
| S
| N
|-
| MboI
| G'''A'''TC
| 24
| S
| N
|-
| MluI
| '''db A'''CGCGT
| 1
| S
| N
|-
| MlyI
| G'''A'''GTC/G'''A'''CTC
| 9
| S
| N
|-
| MunI
| CA'''A'''TTG
| 1
| S
| N
|-
| NlaIII
| C'''A'''TG
| 24
| S
| N
|-
| PaeI
| GC'''A'''TGC
| 1
| S
| N
|-
| PstI
| CTGC'''A'''G
| 0 (2)b
| S
| N
|-
| PvuII
| '''db'''C'''A'''GCTG
| 3
| S
| N
|-
| SspI
| aAT'''A'''TT
| 3
| S
| N
|-
| VspI
| ATTA'''A'''T
| 5
| S
| N
|-
| XbaI
| TCTaG'''A'''
| 1
| S
| N
|-
| XhoI
| CTCG'''A'''G
| 1
| S
| N
|-
| TaiI
| '''A'''CGT
| 16
| S
| N
|-
| TaqI
| TCG'''A'''
| 16
| S
| N
|-
| TfiI
| G'''A'''WTC
| 10
| S
| N
|-
| AluI
| '''A'''GCT
| 24
| S
| P, L
|-
| TasI
| '''A'''ATT
| 28
| S
| P, L
|-
| Tru1I
| TTA'''A'''
| 29
| S
| P, L
|-
| CaiI
| CAGNNNCTG
| 1
| '''U'''
| N
|-
| CseI
| GACGC
| 12
| '''U'''
| N
|-
| Eam1104I
| GAAGAG
| 3
| '''U'''
| N
|-
| Eco147I
| AGGCCT
| 1
| '''U'''
| N
|-
| Eco47III
| AGCGCT
| 3
| '''U'''
| N
|-
| Eco91I
| GGTNACC
| 1
| '''U'''
| N
|-
| LweI
| GCATC
| 24
| '''U'''
| N
|-
| Mph1103I
| ATGCAT
| 3
| '''U'''
| N
|-
| NdeI
| CaTATG
| 1
| '''U'''
| N
|-
| PfeI
| GAWTC
| 10
| '''U'''
| N
|-
| PscI
| ACATGT
| 1
| '''U'''
| N
|-
| SchI
| GAGTC/GACTC
| 9
| '''U'''
| N
|-
| XmiI
| GTMKAC
| 1
| '''U'''
| N
|-
| XapI
| RAATTY
| 6
| '''U'''
| N
|-
| AdeI
| C'''A'''CNNNGTG
| 1
| '''U'''
| N
|-
| BpiI
| GAAGAC
| 5
| '''U'''
| N
|-
| Bsp119I
| TTCGAA
| 1
| '''U'''
| N
|-
| BcnI
| CCSGG
| 12
| (−)
| Y
|-
| BglI
| GGCN5GCC
| 1
| (−)
| Y
|-
| Bme1390I
| CCNGG
| 21
| (−)
| Y
|-
| Bpu1102I
| GCTNAGC
| 2
| '''U'''
| Y
|-
| Bsh1236I
| CGCG
| 37
| (−)
| Y
|-
| Bsp143I
| GATC
| 24
| Not
| Y
|-
| Bsp120I
| GGGCCC
| 1
| (−)
| Y
|-
| Bsp68I
| TCGCGA
| 1
| Not
| Y
|-
| BspLI
| GGNNCC
| 20
| (−)
| Y
|-
| BspOI
| GCTAGC
| 0 (2)b
| '''U'''
| Y
|-
| Bst1107I
| GTATAC
| 1
| '''U'''
| Y
|-
| BsuRI
| GGCC
| 22
| (−)
| Y
|-
| Csp6I
| GTAC
| 3
| '''U'''
| Y
|-
| DpnI
| Gm6ATC
| 24
| Not
| Y
|-
| Eco130I
| CCWWGG
| 1
| (−)
| Y
|-
| Eco88I
| CYCGRG
| 2
| (−)
| Y
|-
| EcoO109I
| RGGNCCY
| 3
| (−)
| Y
|-
| FspBI
| CTAG
| 6
| '''U'''
| Y
|-
| Hin1I
| GRCGYC
| 5
| (−)
| Y
|-
| Hin6I
| GCGC
| 32
| (−)
| Y
|-
| HpyF10VI
| GCN7GC
| 41
| (−)
| Y
|-
| MbiI
| GAGCGG/CCGCTC
| 4
| '''U'''
| Y
|-
| MspI
| CCGG
| 29
| (−)
| Y
|-
| NheI
| GCTAGC
| 0 (2)b
| '''U'''
| Y
|-
| PvuI
| CGATCG
| 1
| Not
| Y
|-
| SmaI
| CCCGGG
| 1
| (−)
| Y
|}
<div>aTested in λ DNA dam- ''in vitro'' methylated with the Hia5 enzyme. bnumber of recognition sites in plasmid pAltOKI.</div>
Y: complete cleavage; N: no digestion; P: partial digestion, L: plasmid linearized; S: sensitive to m6A; U: unknown sensitivity to m6A; Not: not sensitive to m6A; (−): absence of adenine residues in recognition sequence; '''db:''' methylation of both strands required to block cleavage; '''A''': the enzyme will not cut if the '''marked''' adenine is methylated; a: the enzyme is not sensitive to methylation of the marked adenine; A: there are no data on the sensitivity of the enzyme to methylation of the unmarked adenine.

</div>[[Category:Knowledgebase]]

Restriction Methylase - Revision history

Paul.xu at 12:11, 22 June 2017