https://eln.excellgen.com/index.php?action=history&feed=atom&title=RunBlue_Buffer 2026-10-18T05:06:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=RunBlue_Buffer&diff=11934&oldid=prev 2014-06-03T18:42:10Z

<p><span class="autocomment">20x RunBlue SDS Fast Running Buffer</span></p> <p><b>New page</b></p><div>=== 20x RunBlue SDS Running Buffer ===<br /> <br /> &lt;br&gt;0.8 M Tricine&lt;br&gt;1.2 M Tris&lt;br&gt;2% SDS&lt;br&gt;50 mM Sodium Bisulfate <br /> <br /> 71.7g Tricine (free base)&lt;br&gt;72.6g Tris (free base)&lt;br&gt;10.0g SDS&lt;br&gt;2.5g Sodium Bisulfate&lt;br&gt;500mL dd water <br /> <br /> pH 8.2-8.3 at 25C. <br /> <br /> Notes: Add everything but the SDS and fill to ~450mL, make 800mL. <br /> <br /> &lt;br&gt; <br /> <br /> === 1X RunBlue SDS Running Buffer ===<br /> <br /> 20x RunBlue SDS Running Buffer: 40mL <br /> <br /> 800x Anti-oxidant (NXA30010):&amp;nbsp; 1 mL <br /> <br /> ddH2O:&amp;nbsp; 759 mL <br /> <br /> === &lt;br&gt;20x RunBlue SDS &lt;u&gt;Fast &lt;/u&gt;Running Buffer &lt;br&gt; ===<br /> <br /> 0.6 M MOPS&lt;br&gt;1.2 M Tris&lt;br&gt;2% SDS&lt;br&gt;50 mM Sodium Bisulfate – optional (this makes it reducing)<br /> <br /> {| width=&quot;372&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; height=&quot;207&quot;<br /> |-<br /> | MOPS (free acid)<br /> | 62.8g <br /> | &lt;br&gt;<br /> |-<br /> | Tris (free base)<br /> | 72.6g <br /> | &lt;br&gt;<br /> |-<br /> | SDS<br /> | 10.0g <br /> | &lt;br&gt;<br /> |-<br /> | Sodium Bisulfate<br /> | 2.5g <br /> | or 6.5 g&lt;br&gt;<br /> |-<br /> | dd H2O<br /> | 500mL <br /> | &lt;br&gt;<br /> |}<br /> <br /> pH 8.2-8.3 at 25C.&lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Buffer_Recipe]]</div>

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