https://eln.excellgen.com/index.php?action=history&feed=atom&title=RunBlue_Electrophoresis_Buffer 2026-10-18T03:29:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=RunBlue_Electrophoresis_Buffer&diff=786&oldid=prev 2011-05-02T15:57:40Z

<p>Created page with &quot;&#039;&#039;&#039;Tris-Tricine SDS-PAGE Buffer for RUN-Blue Gel&#039;&#039;&#039; RunBlue SDS Running Buffer( cat #: NXB50500)&lt;br&gt;20x Buffer&lt;br&gt;* 0.8 M Tricine&lt;br&gt;* 1.2 M Tris&lt;br&gt;* 2% SDS&lt;br&gt;* 50 mM Sodium B...&quot;</p> <p><b>New page</b></p><div>&#039;&#039;&#039;Tris-Tricine SDS-PAGE Buffer for RUN-Blue Gel&#039;&#039;&#039;<br /> <br /> RunBlue SDS Running Buffer( cat #: NXB50500)&lt;br&gt;20x Buffer&lt;br&gt;* 0.8 M Tricine&lt;br&gt;* 1.2 M Tris&lt;br&gt;* 2% SDS&lt;br&gt;* 50 mM Sodium Bisulfite<br /> <br /> Tricine (free base), 71.70 g<br /> <br /> Tris (free base), 72.60 g<br /> <br /> SDS, 10.00 g<br /> <br /> Sodium Bisulfite, 2.5 g&lt;br&gt;<br /> <br /> Ultra pure water to, 500 ml (~ 385 g)<br /> <br /> The pH should be between 8.2 and 8.3 @ 25°C.<br /> <br /> For Non-reduced samples(especially antibodies), omit the Sodium Bisulfite&lt;br&gt;<br /> <br /> [[Category:Buffer_Recipe]]</div>

Paul.xu