Paul.xu at 15:28, 10 February 2014

2014-02-10T15:28:19Z

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== Laemmli SDS-PAGE buffer ==

 10X electrode buffer stock Tris base 30.3 g Glycine 144.0 g SDS 10.0 g Dissolve in water to a total volume of 1 liter. '''Do not adjust the pH'''. Store at room temperature. To prepare 1X electrode buffer (0.025 M Tris, 0.192 M glycine, 0.1% SDS, pH 8.3), dilute 100 ml of 10X buffer stock with 900 ml of water. 

== Tricine SDS-PAGE buffer ==

 10X electrode buffer stock Tris base 12.1 g Tricine 17.9 g SDS 10.0 g Dissolve in water to a total volume of 1 liter. Do not adjust the pH. Store at room temperature. To prepare 1X electrode buffer (0.1 M Tris, 0.1 M Tricine, 0.1% SDS, pH 8.3), dilute 100 ml of 10X buffer stock with 900 ml of water. 

 

== Laemmli SDS-PAGE buffer ==

 '''Separating Gel Buffer, pH 8.8''' 1.5 M Tris-HCl: 51 g of Tris-HCl,  52.5 g Tris-Base 

4 g SDS Add water to final volume 500 mL, check pH, should be around 8.8 

'''Stacking Gel Buffer, pH 6.8''' 1 M Tris-HCl 78.8 g of Tris-HCl in H2O, 

2 g SDS Final volume 500 mL, check pH, should be around 6.8, if not, adjust with HCl 

 

[[Category:Buffer_Recipe]]

SDS-PAGE Buffers - Revision history

Paul.xu at 15:28, 10 February 2014