Paul.xu at 14:53, 28 December 2019

2019-12-28T14:53:13Z

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'''SOC Medium'''

0.5% yeast extract 2% tryptone 10 mM NaCl 2.5 mM KCl 10 mM MgCl2 10 mM MgSO4 and 20 mM glucose.
<pre>1.Add the following to 90ml of distilled H2O
2g Bacto Tryptone
0.5 g Bacto Yeast Extract
0.25 ml of 4M NaCl.
0.25 ml of 1M KCl.
1 ml of 1M MgCl2
1 ml of 1M MgSO4, optional
2 ml of 1M glucose

2. Adjust to 100mL with distilled H2O

3. Sterilize by autoclaving
</pre>

or

500 ml '''SOC Medium''' ● 1.802g glucose ● 10g tryptone ● 2.5g Yeast extract ● 0.584g NaCl ● 0.093g KCl ● 1.016g MgCl2 (anhydrous) ○ Note: If you don’t have MgCl2 (anhydrous), use 2.170g of MgCl26H20 ● 1.234g MgSO4.7H2O ● 500ml of dH20 (distilled water)

'''SOB Medium''' 0.5% (w/v) yeast extract 2% (w/v) tryptone 10 mM NaCl 2.5 mM KCl 20 mM MgSO4

Per liter:

5 g yeast extract 20 g tryptone 0.584 g NaCl 0.186 g KCl 2.4 g MgSO4 Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
[[Category:Buffer Recipe]]

SOC Medium - Revision history

Paul.xu at 14:53, 28 December 2019