https://eln.excellgen.com/index.php?action=history&feed=atom&title=Signal_peptide_comparison
Signal peptide comparison - Revision history
2026-10-18T07:56:04Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Signal_peptide_comparison&diff=7989&oldid=prev
Paul.xu at 21:25, 26 January 2013
2013-01-26T21:25:43Z
<p></p>
<p><b>New page</b></p><div>Transfection: <br> <br />
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5 ul&nbsp; (100 ~ 200 ng/ul) from miniprep, each well is from individuale clone<br> <br />
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24 well plate.<br> <br />
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1393: pCMV5-IL2-TagGFP-(3c)-Fc-WPRE-OriP<br> <br />
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1394: pCMV5-CST4-TagGFP-Fc(3c)-WPRE-OriP<br> <br />
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1395: pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP <br> <br />
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1396: pCMV5-hIgG-Hc-TagGFP-Fc(3c)-WPRE-OriP<br> <br />
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[[Image:GFP-comparison 2012 10 18.jpg]] <br />
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Nov 2012 <br />
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Transfect with L2K. 1433 plasmid DNA quality is low, only 20 ng/ul<br>[[Image:Secretion signal comparison 2012 11 20.jpg]]<br> <br />
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[[Image:Signal peptide comp 2012 11 22.jpg]] <br />
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2012/11/23 <br />
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[[Image:Signal peptides 2012 11 23 1.jpg]] <br />
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11/24/2012 <br />
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[[Image:Signal peptides 2012 11 24 1.jpg]] <br />
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[[Image:Signal peptides 2012 11 25.jpg]] <br />
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11/20/2012: Transfection using lipofectamine 2000, 1 ug DNA, 3 ul L2K, 12 well plate<br> <br />
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Picture on 2012/11/22<br> <br />
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[[Image:Signal peptide L2K 2012 11 22.jpg]]<br> <br />
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Picture on 2012/11/23<br> <br />
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[[Image:Signal peptides 2012 11 23 2.jpg]] <br />
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11/24/2012 <br />
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[[Image:Signal peptides 2012 11 24 2 1.jpg]] <br />
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2012/11/25 <br />
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[[Image:Signal peptides 2012 11 25 2.jpg]][http://www.excellgen.net/eln/images/4/4e/Signal_seq_comparison_2013_1_24.jpg Signal_seq_comparison_2013_1_24.jpg] <br />
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11/21/2012: Transfection using GeneExpression Max<br> <br />
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1 ug DNA, 3 ul GEM, 12 well plate<br> <br />
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Picture on 11/22/2012 <br />
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[[Image:Signal peptides GEM 2012 11 12.jpg]] <br />
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[[Image:Signal peptides 2012 11 23 3.jpg]] <br />
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2012/11/24 <br />
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[[Image:Signal peptides 2012 11 24 3 2.jpg]] <br />
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2012/11/25 <br />
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[[Image:Signal peptide comp 2012 11 25.jpg]] <br />
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2012/12/12 <br />
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transfection on 2012/12/11: 1 ug/well, 3 ul GEM, picture taken on 2012/12/12 <br />
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[[Image:Signal peptide comparison 2012 12 12.jpg]] <br />
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2012/12/13 <br />
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[[Image:Signal peptide comparison 2012 12 13.jpg]] <br />
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2012/12/14 <br />
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[[Image:Signal peptide comparison 2012 12 14.jpg]] <br />
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plate <br />
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[[Image:Signal peptide comparison plate 2012 12 14.jpg]] <br />
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= Compare new IL2 signal peptide vs other =<br />
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12 well plate, 293T cells <br />
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Transfection: 1 ug DNA <br />
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Transfection reagent: GEM <br />
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Date: 2013/1/24 <br />
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2013 1 25 <br />
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[[Image:Signal seq comparison 2013 1 24.jpg]] <br />
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= [[Image:Signal seq comparison 2013 1 25.jpg]]<br> =<br />
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2013/1/26<br />
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[[Image:Signal_seq_comparison_2013_1_26.JPG]]<br />
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[[Category:QC]] [[Category:293_Expression]]</div>
Paul.xu