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Subcloning Tasks - Revision history
2026-10-18T04:57:10Z
Revision history for this page on the wiki
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Crm at 22:00, 19 April 2018
2018-04-19T22:00:56Z
<p></p>
<p><b>New page</b></p><div>{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| <br/><br />
| '''ID'''<br />
| '''Name'''<br />
| '''DNA Size'''<br />
| '''Vector'''<br />
| '''Parent'''<br />
| '''Method'''<br/><br />
| '''Date'''<br />
| '''Enzyme'''<br/><br />
| '''Expression'''<br />
| '''Note'''<br />
|-<br />
| 0<br />
| 3161<br/><br />
| &nbsp;pET28b-GST--Myc-HA-Flag-V5<br/><br />
| 224<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;">433 pDB.His.GST</span><br/><br />
| 3159&nbsp;<span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pMiniT2-1FRG-HA-VL-kappa</span><br/><br />
| &nbsp;<br />
| <br/><br />
| Nde1-Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">3127</span><br/><br />
| <span style="color:#FF0000;">pET28b-qBeta-qBeta-6XHis</span><br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <span style="color:#FF0000;">PCR qbeta and clone back into 3097</span><br />
| <br/><br />
| <span style="color:#FF0000;">Nde1-HindIII</span><br />
| <span style="color:#FF0000;">C-His tag</span><br />
| <span style="color:#FF0000;">PCR product is in pT&A3, 997, sequenced.</span>&nbsp;<br />
|-<br />
| <br/><br />
| 812<br/><br />
| &nbsp;pET30b-phoA<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <span style="font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">3074</span><br/><br />
| <span style="font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pET30b-HA-avitag-BirA</span><br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| in pJet1.2 is good, subclone into pET28b, no his tag, need to subclone into pT7T30-Avi-BirA (still no his tag)<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">938</span><br />
| <span style="color:#FF0000;">PNGase F,&nbsp;</span><br />
| <span style="color:#FF0000;">1145</span><br />
| <span style="color:#FF0000;">pET30b</span><br/><br />
| <span style="color:#FF0000;">Nde1-Xho1</span><br />
| <span style="color:#FF0000;">pJet1.2, sequencing is good, need to subclone into pET30b</span><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br />
<span style="color:#FF0000;">C-His</span><br />
<br />
[[938_pET30b-PNGase_F|<span style="color:#FF0000;">result</span>]]<br />
<br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">939</span><br />
| <span style="color:#FF0000;">Tte-Exo III</span><br />
| <span style="color:#FF0000;">780</span><br />
| <span style="color:#FF0000;">pET30b</span><br/><br />
| <span style="color:#FF0000;">Nde1-Xho1</span><br/><br />
| <br />
<span style="color:#FF0000;">pJet1.2</span><br />
<br />
<span style="color:#FF0000;">sequencing is good, need to subclone into pET30b</span><br />
<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br />
<span style="color:#FF0000;">C-His</span><br />
<br />
[[939_pET30b-Tte-ExoIII|<span style="color:#FF0000;">result</span>]]<br />
<br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">942</span><br />
| <span style="color:#FF0000;">VVN endonuclease</span><br />
| <span style="color:#FF0000;">716</span><br />
| <span style="color:#FF0000;">pET28b-pMBP</span><br/><br />
| <span style="color:#FF0000;">Nco1-Xho1</span><br />
| <br />
<span style="color:#FF0000;">subclone from 942-3-PJET1-2</span><br />
<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br />
<span style="color:#FF0000;">C-H</span>is<br />
<br />
[[942_pET28b-pMBP-VVN|result]]<br />
<br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">3122</span><br />
| <span style="color:#FF0000;">Crm197, secreted</span><br />
| <span style="color:#FF0000;">1743</span><br />
| <span style="color:#FF0000;">pET30b</span><br/><br />
| <span style="color:#FF0000;">Nde1-Xho1</span><br/><br />
| <span style="color:#FF0000;">pJet1.2</span><br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <span style="color:#FF0000;">C-His</span><br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">931</span><br />
| <span style="color:#FF0000;">PAK16D ligase</span><br />
| <span style="color:#FF0000;">2267</span><br />
| <span style="color:#FF0000;">pET28b</span><br />
| <span style="color:#FF0000;">Nde1-HindIII-Xho1</span><br/><br />
| <br />
<span style="color:#FF0000;">pJet1.2</span><br />
<br />
<span style="color:#FF0000;">fragment 2 is good</span><br />
<br />
<span style="color:#FF0000;">fragment 1&nbsp; is also good</span><br />
<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <span style="color:#FF0000;">N-H</span>is<br />
|-<br />
| <br/><br />
| 945<br/><br />
| pT7A30-pMBP<br/><br />
| <br/><br />
| pT7A3<br />
| Xba1-Nco1<br/><br />
| PCR from&nbsp;1281 pMAL-p5E or 1282 pMAL-p5E-AvPAL&nbsp;<br />
| <br/><br />
| <br/><br />
| <br/><br />
| [[945_pT7A30-pMBP|result]]<br />
|-<br />
| <br/><br />
| 947<br/><br />
| pT7A30-6His-pMBP<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| [[947_pT7A30-6His-pMBP|result]]<br />
|-<br />
| <br/><br />
| 949<br/><br />
| pET28b-GST-sfGFP-Myc-HA-Flag-V5<br/><br />
| <br/><br />
| 433 546<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <span style="color:#FF0000;">824</span><br/><br />
| <span style="color:#FF0000;">pET28b-VVTopo</span><br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| 985<br/><br />
| pCold-GST-3C-AP205-M13-g3p<br/><br />
| <br/><br />
| 2047&nbsp; pCold-C6H<br/><br />
| 983&nbsp;pMiniT2-973-2<br/><br />
| insert: Nde1-Sal1, vector: Nde1-Xho1<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| [[985_pCold-GST-3C-AP205-M13-g3p|Result]]<br />
|-<br />
| <br/><br />
| 984<br/><br />
| pT7A3-6H-SBP-SUMO-3C<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| 986&nbsp;<br/><br />
| pT7A3-6His-NLS-Cas9-NLS-6xHis<br/><br />
| <br/><br />
| <br/><br />
| need to sequencing, expression screening<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| [[986_pT7A3-6His-NLS-Cas9-NLS-6xHis|result]]<br />
|}<br />
<br />
== ==<br />
[[Category:To Do]] [[Category:Tasks]]</div>
Crm