https://eln.excellgen.com/index.php?action=history&feed=atom&title=Subramanian_1_shRNA Subramanian 1 shRNA - Revision history 2026-10-18T02:24:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Subramanian_1_shRNA&diff=187&oldid=prev Ztao at 14:53, 4 February 2011 2011-02-04T14:53:35Z <p></p> <p><b>New page</b></p><div>Notebook: Zhen Tao #100&lt;br&gt; <br /> <br /> Page: 001910&lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No1.JPG]]&lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No2.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No3.JPG]]&lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No4.JPG]]&lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No5.JPG]]&lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA day2 after transfection No6.JPG]]&lt;br&gt; <br /> <br /> [[Image:Subramanian_1_shRNA_day2_after_transfection_No7.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 551px; height: 203px;&quot;<br /> |-<br /> | plate number&lt;br&gt; <br /> | plasmid&lt;br&gt; <br /> | packaging mix&lt;br&gt; <br /> | transfection reagent&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | PNPLA2 367881 &lt;br&gt; <br /> | 108 ul (V:G, 1:3)&lt;br&gt; <br /> | BPEI25&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | PNPLA2 367881&lt;br&gt; <br /> | 108 ul (V:G, 1:3)&lt;br&gt; <br /> | PEIMAX&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | PNPLA2 367882&lt;br&gt; <br /> | 108 ul (V:G, 1:3)&lt;br&gt; <br /> | BPEI25&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | PNPLA2 367882&lt;br&gt; <br /> | 108 ul (V:G, 1:3)&lt;br&gt; <br /> | PEIMAX&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | PNPLA2 367881&lt;br&gt; <br /> | 75.6 ul (V:G, 1:3)&lt;br&gt; <br /> | BPEI25&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | PNPLA2 367882&lt;br&gt; <br /> | 75.6 ul (V:G, 1:3)&lt;br&gt; <br /> | BPEI25&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | PNPLA2 367882&lt;br&gt; <br /> | 75.6 ul (V:G, 1:3)&lt;br&gt; <br /> | PEIMAX&lt;br&gt;<br /> |}<br /> <br /> --[[User:Ztao|Ztao]] 09:40, 4 February 2011 (EST) &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> PEIMAX or BPEI25: 32.4 ul/15cm plate, or 22.68 ul/square plate&lt;br&gt; <br /> <br /> PNPLA2: 7.2 ug/15 cm plate or 5 ug/square plate&lt;br&gt; <br /> <br /> Packaging mix: 108 ul/15cm plate or 75.6 ul/square plate (1:3, pVSVG/pGag-Pol) <br /> <br /> --[[User:Ztao|Ztao]] 17:00, 28 January 2011 (EST) --[[User:Ztao|Ztao]] 10:50, 3 February 2011 (EST) <br /> <br /> [[Category:Lentivirus]]</div> Ztao