https://eln.excellgen.com/index.php?action=history&feed=atom&title=Subramanian_1_shRNA-2 2026-10-18T14:10:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Subramanian_1_shRNA-2&diff=259&oldid=prev 2011-02-10T14:15:41Z

<p></p> <p><b>New page</b></p><div>Notebook: Zhen Tao <br /> <br /> Page: 001922 <br /> <br /> &lt;br&gt; <br /> <br /> Transfection reagent: PEIMAX, 32.4 ul/flask <br /> <br /> Packaging Mix: V:G = 1:3 (prepared by mixing 0.1 ug/ul corresponding DNA). 108 ul/flask <br /> <br /> Plasmid (PNPLA2_216390 or PNPLA2_216192): 7.2 ug/flask. <br /> <br /> Use 1 ml DMEM(-) to prepare transfection reagent or plasmid, then add transfection reagent to plasmid and keep at room temperature for 20 min; add it to flask drop by drop. <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2 216390 PEIMAX day1 after transfection.JPG|391x235px|Subramanian 1 shRNA PNPLA2 216390 PEIMAX day1 after transfection.JPG]] <br /> <br /> 1 day after transfection <br /> <br /> --[[User:Ztao|Ztao]] 14:00, 8 February 2011 (EST) <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216192-1 PEIMAX day2 after transfection.JPG|391x220px|Subramanian 1 shRNA PNPLA2-216192-1 PEIMAX day2 after transfection.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216192-2 PEIMAX day2 after transfection.JPG|384x216px|Subramanian 1 shRNA PNPLA2-216192-2 PEIMAX day2 after transfection.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216390-1 PEIMAX day2 after transfection.JPG|384x229px|Subramanian 1 shRNA PNPLA2-216390-1 PEIMAX day2 after transfection.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216390-2 PEIMAX day2 after transfection.JPG|385x219px|Subramanian 1 shRNA PNPLA2-216390-2 PEIMAX day2 after transfection.JPG]] <br /> <br /> 2 day after transfection --[[User:Ztao|Ztao]] 11:58, 9 February 2011 (EST) <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216192-1 PEIMAX day3 after transfection.JPG|381x230px|Subramanian 1 shRNA PNPLA2-216192-1 PEIMAX day3 after transfection.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216192-2 PEIMAX day3 after transfection.JPG|379x228px|Subramanian 1 shRNA PNPLA2-216192-2 PEIMAX day3 after transfection.JPG]] <br /> <br /> [[Image:Subramanian 1 shRNA PNPLA2-216390-1 PEIMAX day3 after transfection.JPG|379x230px|Subramanian 1 shRNA PNPLA2-216390-1 PEIMAX day3 after transfection.JPG]] <br /> <br /> [[Image:Subramanian_1_shRNA_PNPLA2-216390-2_PEIMAX_day3_after_transfection.JPG|378x228px]]<br /> <br /> 3 day after transfection --[[User:Ztao|Ztao]] 09:15, 10 February 2011 (EST)<br /> <br /> [[Category:Lentivirus]]</div>

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