Paul.xu: Created page with "10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20 SYBR-G 1:1000 2.5 µl 1U
Hotstar Taq 0...."

2019-09-23T13:30:31Z

Created page with "10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20 SYBR-G 1:1000 2.5 µl 1U Hotstar Taq 0...."

New page

10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20

SYBR-G 1:1000 2.5 µl 1U  Hotstar Taq 0.5 µl 2,5 U  Rox (10 µM) 0.33 µl 0,1333 µM  MgCl2 (25mM) 1 µl 1mM  dNTPs 10mM 0.5 µl 200µM  Triton X-100 0.375 µl 1.50%  DMSO 1.25 µl 5% 
[[Category:Protocols]]

Sybr Green Mix - Revision history

Paul.xu: Created page with "10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20 SYBR-G 1:1000 2.5 µl 1U Hotstar Taq 0...."

Paul.xu: Created page with "10 X PCR Buffer: 120 mM Tris-HCl, pH 8.3, 500 mM KCl, 50 mM MgCl2, 1.5 M Trehalose, 1M Betaine, 2 mg/ml BSA, 2% v/v Tween-20 SYBR-G 1:1000 2.5 µl 1U
Hotstar Taq 0...."