https://eln.excellgen.com/index.php?action=history&feed=atom&title=Synthesis_AEPZ-CBA 2026-10-18T03:27:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Synthesis_AEPZ-CBA&diff=2430&oldid=prev 2011-11-18T14:42:32Z

<p></p> <p><b>New page</b></p><div>synthesis of reducible hyperbranched poly(amidoamine) (RHB)&lt;br&gt; <br /> <br /> Michael addition copolymerization&amp;nbsp; of half molar ratio of a triamine AEPZ-to-bisacrylamide monomers (CBA and MBA).&lt;br&gt; <br /> <br /> [[Image:1-methypiperazine.png]]&lt;br&gt; <br /> <br /> 1. Weigh AEPZ (0.193 g, 1.5 mmol), add to methanol/water mixture (3.5 mL, 7/3 v/v) in a small vial.&lt;br&gt; <br /> <br /> 2. Add CBA (0.260 g, 1.0 mmol) and MBA (0.308 g, 2.0 mmol) to vial.&lt;br&gt;&lt;br&gt; <br /> <br /> 3. React at 50 °C for 5 days, yielding a viscous solution.&lt;br&gt; <br /> <br /> 4. Add small amount of 1-methylpiperazine &amp;nbsp; to consume any unreacted acrylamide groups.&lt;br&gt; <br /> <br /> 5. Stir for 12 h at 50 °C.&lt;br&gt; <br /> <br /> 6. Add cool acetone to precipitate polymer. <br /> <br /> 7. Dry precipitate under vacuum at room temperature.&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Transfection]] [[Category:Paul]]</div>

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