https://eln.excellgen.com/index.php?action=history&feed=atom&title=Synthesis_Chitosan_PEI 2026-10-18T08:37:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Synthesis_Chitosan_PEI&diff=12483&oldid=prev 2014-07-21T19:37:35Z

<p>Created page with &quot;== Reagents&lt;br&gt; == Chitosan: Mw = 50 kDa and deacetylation degree = 90%&lt;br&gt; 1,19-carbonyldiimidazole &lt;br&gt; &lt;br&gt; == Synthesis == <a href="/index.php?title=File:Chitosan_PEI_Conjugation.jpg" title="File:Chitosan PEI Conjugation.jpg">Image:Chitosan_PEI_Conjugation.jpg</a> Copoly...&quot;</p> <p><b>New page</b></p><div>== Reagents&lt;br&gt; ==<br /> <br /> Chitosan: Mw = 50 kDa and deacetylation degree = 90%&lt;br&gt;<br /> <br /> 1,19-carbonyldiimidazole &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> == Synthesis ==<br /> <br /> [[Image:Chitosan_PEI_Conjugation.jpg]]<br /> <br /> Copolymer of CP was prepared according to the methods described by Gao. &lt;br&gt;<br /> <br /> Dissolve 0.5 g CS in 20 ml of a 0.5% acetic acid solution &lt;br&gt;<br /> <br /> adjust to pH 7.0 &lt;br&gt;<br /> <br /> Stir the mixture overnight. &lt;br&gt;<br /> <br /> Add CDI at a molar ratio of CDI:CS amine = 2:1 &lt;br&gt;<br /> <br /> Stir for 1 h. &lt;br&gt;<br /> <br /> Add PEI (1.8 kDa) dropwise into the solution under stirring at a molar ratio of PEI:amine = 2:1.&lt;br&gt;<br /> <br /> Polymerize at room temperature (25 oC) overnight. &lt;br&gt;<br /> <br /> Dialyse in water for 48 h.&lt;br&gt;<br /> <br /> Lyophilize <br /> <br /> [[Category:Paul]] [[Category:Transfection]]</div>

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