https://eln.excellgen.com/index.php?action=history&feed=atom&title=Synthesis_HBPAA 2026-10-18T09:32:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Synthesis_HBPAA&diff=2448&oldid=prev 2011-11-18T17:53:00Z

<p>Created page with &quot;Hyperbranched poly(amido amine)s synthesized by Michael addition copolymerization by reacting equal molar ratio of a triamine (DMDPTA) and bisacrylamide monomers (CBA and HMBA). ...&quot;</p> <p><b>New page</b></p><div>Hyperbranched poly(amido amine)s synthesized by Michael addition copolymerization by reacting equal molar ratio of a triamine (DMDPTA) and bisacrylamide monomers (CBA and HMBA). <br /> <br /> 1. Add CBA (0.260 g, 1.0 mmol) or/and HMBA (0.154 g, 1.0 mmol) to a small vial containing DMDPTA (0.318 g, 2.0 mmol) in methanol/water mixture (3.5 mL, 8/2 v/v). &lt;br&gt;2. React for 3 days at 50 °C. &lt;br&gt;3. Add additional 0.1 mmol of DMDPTA to the reaction mixture to consume any residual acrylamide groups, and stir for 12 h at 50 °C. &lt;br&gt;4. Dialysis against distilled water acidified with HCl to pH 3, then water&lt;br&gt;5. Recover polymer by freeze drying.&lt;br&gt;6. Typical yield after dialysis was 30–40%.<br /> <br /> N,N′-cystaminebisacrylamide (CBA)<br /> <br /> N,N′-hexamethylenebisacrylamide (HMBA) &lt;br&gt;N,N-dimethyldipropylenetriamine (DMDPTA, Aldrich)<br /> <br /> [[Image:RHB_synthesis.png]]&lt;br&gt;&lt;br&gt;<br /> <br /> <br /> <br /> [[Image:RHB_efficiency.png]]<br /> <br /> <br /> <br /> [[Image:RHB_chloroquine.png]]<br /> <br /> <br /> <br /> [[Image:RHB_cell_viability.png]]<br /> <br /> [[Category:Transfection]][[Category:Paul]]</div>

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