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Synthesis PHP-CBA-DSP-PEI18 SOP - Revision history
2026-10-18T08:06:53Z
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Paul.xu: Created page with "== Synthesis PHP-DSP-PEI SOP == PEI18: 4g/6 ml H2O (666 mg/ml) 1.5 g solid 1,3-Diamino-2-propanol, MW 90.12, 16.6 mmole +3 ml MeOH +3.63 g 1,3-dibrono-2-propanol&n..."
2016-06-27T20:56:36Z
<p>Created page with "== Synthesis PHP-DSP-PEI SOP == PEI18: 4g/6 ml H2O (666 mg/ml) 1.5 g solid 1,3-Diamino-2-propanol, MW 90.12, 16.6 mmole +3 ml MeOH +3.63 g 1,3-dibrono-2-propanol&n..."</p>
<p><b>New page</b></p><div>== Synthesis PHP-DSP-PEI SOP ==<br />
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PEI18: 4g/6 ml H2O (666 mg/ml)<br />
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1.5 g solid 1,3-Diamino-2-propanol, MW&nbsp; 90.12, 16.6 mmole<br />
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+3 ml MeOH<br />
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+3.63 g 1,3-dibrono-2-propanol&nbsp;, MW&nbsp; 217.89, 16.7 mmole<br/>50 oC for 48 hours<br />
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store at -20 oc.<br />
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add 5 ml H2O to dissolve<br />
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+ 25 ml EtOH, mix, spin 3000 rpm for 5 min<br />
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remove supernatant<br />
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Add 5 ml H2O, + 25 ml EtOH<br />
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spin, repeat 2 more times<br />
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weigh wet pellet, calculate yield (usually 2.8 g wet PHP)<br />
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Add H2O to 10 ml to dissolve<br />
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Add 83 mg PEI18, & 83 mg DSP, reaction at RT o/n<br />
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Paul.xu