Paul.xu at 14:35, 27 June 2014

2014-06-27T14:35:52Z

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== Comparison of DTBP vs DSP and PEI800 vs PEI18 ==

0.4 mg/ul PEI10 in DMSO

6/22/14

{| width="200" cellspacing="1" cellpadding="1" border="1"
|-
| Sample #
| PHP suspension
| PEI800-DTBP
| PEI800-DSP
| PEI1800
|
DSP

100mg/ml

|
DTBP

100mg/ml

| Total
|-
| 1
| 50
| 
|
| 2.5
| 5
|
|
|-
| 2
| 50
| 
|
| 5
| 7.5
| 
|
|-
| 3
| 50
|
| 
| 7.5
| 10
|
|
|-
| 4
| 50
|
| 5
|
| 5
| 
|
|-
| 5
| 50
|
| 10
| 
| 7.5
|
|
|-
| 6
| 50
|
| 15
| 
| 10
| 
|
|-
| 7
| 50
| 7.5*
|
|
| 5
|
|
|-
| 8
| 50
| 10
|
|
| 7.5
| 
|
|-
| 9
| 50
| 15
| 
|
| 10
|
|
|-
| 10
| 50
|
| 
| 2.5
|
| 5
|
|-
| 11
| 50
|
|
| 5
|
| 7.5
|
|-
| 12
| 50
|
|
| 7.5
|
| 10
|
|-
| 13
| 50
|
| 5
|
|
| 5
|
|-
| 14
| 50
|
| 10
|
|
| 7.5
|
|-
| 15
| 50
|
| 15
|
|
| 10
|
|-
| 16
| 50
| 7.5*
|
|
|
| 5
|
|-
| 17
| 50
| 10
|
|
|
| 7.5
|
|-
| 18
| 50
| 15
|
|
|
| 10
|
|}

*add 7.5 ul because 5 ul is not enough to form soluble PHP-PEI800 complex

 

React RT o/n

6/23/14

Dilute 1 ul in 30 ul  10 mM Hepes, pH 7.0

use 3 ul to transfect 1 ug DNA, 293T cells.

6/24/14, take pictures

[[Image:6 24 14 DSP DTBP.JPG]]

 

[[Image:PHP-DTBP-DSP comp 6 26 14.JPG]]

[[Image:PHP-DTBP-DSP_comp_6_27_14.JPG]]

[[Category:Transfection]] [[Category:Paul]]

Synthesis PHP-DTBP-DSP Comparison - Revision history

Paul.xu at 14:35, 27 June 2014