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Synthesis PHP-S-S-PEI18 - Revision history
2026-10-18T03:29:51Z
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Paul.xu at 18:10, 21 July 2014
2014-07-21T18:10:39Z
<p></p>
<p><b>New page</b></p><div>== Conjugate PHP tp PEI18 without DSP ==<br />
<br />
{| width="703" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| # <br />
| PHP <br />
| 0.4g ml/mlPEI <br />
| DBP <br />
| Cystamine <br />
| Observation<br><br />
|-<br />
| 1 <br />
| 50ul <br />
| 5 ul <br />
| 0 <br />
| 0 <br />
| smallest pellet after isopropanol ppt<br><br />
|-<br />
| 2 <br />
| 50 <br />
| 5 <br />
| 1 <br />
| 0 <br />
| <br><br />
|-<br />
| 3 <br />
| 50 <br />
| 5 <br />
| 2 <br />
| 0 <br />
| <br><br />
|-<br />
| 4 <br />
| 50 <br />
| 5 <br />
| 3 <br />
| 0 <br />
| <br><br />
|-<br />
| 5 <br />
| 50 <br />
| 5 <br />
| 1 <br />
| 5 ul <br />
| large white pellet after isopropanol ppt<br><br />
|-<br />
| 6 <br />
| 50 <br />
| 5 <br />
| 2 <br />
| 5 <br />
| largest white pellet after isopropanol ppt<br><br />
|-<br />
| 7 <br />
| 50 <br />
| 5 <br />
| 3 <br />
| 5 <br />
| pellet smaller than #5<br><br />
|}<br />
<br />
react o/n at 50 oC.purify by isopropanol ppt. dissolve in DMSO.<br> <br />
<br />
7/16/14: make 1:10, 1:30 dilution using pH7.0 HEPES buffer, 10 mM for transfection, 1 ug DNA + 3 ul. 293 T <br />
<br />
7/18/14: picture after 2 days. <br />
<br />
[[Image:PHP GU PEI no DSP 7 18 14.JPG]] <br />
<br />
7/19/14 <br />
<br />
[[Image:PHP GU PEI no DSP 7 19 14.JPG]] <br />
<br />
7/20/14 <br />
<br />
[[Image:PHP GU PEI no DSP 7 20 14.JPG]] <br />
<br />
7/21/14<br />
<br />
[[Image:PHP_GU_PEI_no_DSP_7_21_14.JPG]]<br />
<br />
== Conclusion ==<br />
<br />
Without DSP conjugation the transfection efficiency is low <br />
<br />
[[Category:Paul]] [[Category:Transfection]]</div>
Paul.xu