Paul.xu at 14:03, 1 December 2011

2011-12-01T14:03:48Z

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0.75 g 1,3-diamino-2-propanol (solid), 8.4 mmole 

+ 1.5 ml MeOh to dissolve 

#1 + 50 mg CBA

#2 + 100 mg CBA

+ 1.84 g 1, 3-dibromo-2-propanol liquid (~ 1ml), 8.4 mmole 

react at 50 oc for 2 days. 

+ 5 ml H2O to dissolve, +30 ml EtOH, + 10 ml isopropano, spin, remove solvent. wash again. 

 to #1, + 60 mg dsp + 40 mg PEI12 

to #2 + 40 mg dsp + 40 mg PEI12 

react at 37 oC o/n. 

 

resulting product: 

#1: PHPCBA50-DSP-PE

#2: PHPCBA100-DSP-PEI

transfection, 1 ug DNA, 3 ul diluted polymer 

Plate 1 

{| cellspacing="1" cellpadding="1" border="1" style="width: 566px; height: 81px;"
|-
| PHP-DSP-PEI18, 1:10 
| PHP-DSP-PEI18, 1:20 
| PHP-DSP-PEI18, 1:30 
| PHP-DSP-PEI18, 1:40 
|-
| PHPCBA50-DSP-PEI, 1:10 
| PHPCBA50-DSP-PEI, 1:20 
| PHPCBA50-DSP-PEI, 1:30 
| PHPCBA50-DSP-PEI, 1:40 
|-
| PHPCBA100-DSP-PEI, 1:10 
| PHPCBA100-DSP-PEI, 1:20 
| PHPCBA100-DSP-PEI, 1:30 
| PHPCBA100-DSP-PEI, 1:40 
|}

picture 16 hr after transfection (picture taken at 9AM on Nov, 28, 2011, transfection: 5PM on Nov 27, 2011) [[Image:Plate1 2011 11 28.jpg]]

11/29/2011

[[Image:PHPCBA-DSP-PEI12 2011 11 29 2.jpg]]

 

11/30/2011

[[Image:Plate1 2011 11 30.jpg]]

2011/12/1 

[[Image:Plate1 2011 12 1.jpg]]

Plate 2

{| cellspacing="1" cellpadding="1" border="1" style="width: 566px; height: 81px;"
|-
| PHP-DSP-PEI18, 1:50 
| PHP-DSP-PEI18, 1:60 
| PHP-DSP-PEI18, 1:70 
| PHP-DSP-PEI18, 1:80 
|-
| PHPCBA50-DSP-PEI, 1:50 
| PHPCBA50-DSP-PEI, 1:60 
| PHPCBA50-DSP-PEI, 1:70 
| PHPCBA50-DSP-PEI, 1:80 
|-
| PHPCBA100-DSP-PEI, 1:50 
| PHPCBA100-DSP-PEI, 1:60 
| PHPCBA100-DSP-PEI, 1:70 
| PHPCBA100-DSP-PEI, 1:80 
|}

picture 16 hr after transfection (picture taken at 9AM on Nov, 28, 2011, transfection: 5PM on Nov 27, 2011) [[Image:Plate2 2011 11 28.jpg]] 

11/29/2011

[[Image:PHPCBA-DSP-PEI12 2011 11 29 1.jpg]]

2011/11/30 

[[Image:Plate2 2011 11 30.jpg]]

2011/12/1 

[[Image:Plate2 2011 12 1.jpg]]

Plate 3

{| cellspacing="1" cellpadding="1" border="1" style="width: 566px; height: 81px;"
|-
| PHP-DSP-PEI18, 1:10 
| PHP-DSP-PEI18, 1:20 
| PHP-DSP-PEI18, 1:30 
| PHP-DSP-PEI18, 1:40 
|-
| PHPCBA50-DSP-PEI, 1:10 
| PHPCBA50-DSP-PEI, 1:20 
| PHPCBA50-DSP-PEI, 1:30 
| PHPCBA50-DSP-PEI, 1:40 
|-
| PHPCBA100-DSP-PEI, 1:10 
| PHPCBA100-DSP-PEI, 1:20 
| PHPCBA100-DSP-PEI, 1:30 
| PHPCBA100-DSP-PEI, 1:40 
|}

picture 16 hr after transfection (picture taken at 9AM on Nov, 28, 2011, transfection: 5PM on Nov 27, 2011) [[Image:Plate3 2011 11 28.jpg]] 

11/29/11

[[Image:PHPCBA-DSP-PEI12 2011 11 29 3.jpg]]

2011/11/30 

[[Image:Plate3 2011 11 30.jpg]]

2011/12/1 

[[Image:Plate3 2011 12 1.jpg]]

Conclusion: Add CBA to polymer synthesis reaction will decrease transfection efficiency. 

[[Category:Transfection]] [[Category:Paul]]

Synthesis PHPCBA-DSP-PEI12 - Revision history

Paul.xu at 14:03, 1 December 2011