https://eln.excellgen.com/index.php?action=history&feed=atom&title=Synthesis_Thiolated_Chitosan 2026-10-18T07:55:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Synthesis_Thiolated_Chitosan&diff=12495&oldid=prev 2014-07-23T17:27:05Z

<p>Created page with &quot;Chitosan-cysteine&lt;br&gt;conjugates synthesized by the addition of 50 mM EDAC has the highest content of thiol&lt;br&gt;groups of 265,169 μmol per 50 mg of conjugates.&lt;br&gt; &lt;br&gt; Synthesi...&quot;</p> <p><b>New page</b></p><div>Chitosan-cysteine&lt;br&gt;conjugates synthesized by the addition of 50 mM EDAC has the highest content of thiol&lt;br&gt;groups of 265,169 μmol per 50 mg of conjugates.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> Synthesis of chitosan-cysteine conjugates&lt;br&gt;Chitosan-cysteine conjugates are made by: prepared beaker glass capacity 500 mL of&lt;br&gt;4 pieces. Made of 1% acetic acid solution of 250 mL in each beaker. Then into each beaker&lt;br&gt;glass was dissolved 5 g of chitosan. After all the soluble chitosan, into each beaker glass is&lt;br&gt;added 2,5 g of cysteine until dissolved. Then added with a concentration 0 mM EDAC, 30&lt;br&gt;mM, 40 mM, and 50 mM in each solution. pH value adjusted to 5 with the addition of NaOH&lt;br&gt;1 N. The mixture was incubated for 3 hours with continous stirring. The solution was&lt;br&gt;dialyzed twice in a dialysis tubing (MW cut-off 12-14 kDa) protected from light at 100C with&lt;br&gt;1 mM HCl containing 1% NaCl for 1 day and then with 1 mM HCl for 2 days to eliminate&lt;br&gt;EDAC and cysteine of the conjugate solution. The solution is lyophilized and conjugates (dry&lt;br&gt;conditions) maintained at 4oC until ready for use (Roldo et al., 2004).&lt;br&gt;<br /> <br /> [[Category:Paul]][[Category:Transfection]]</div>

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