Paul.xu: Created page with "1. Disolve oligoarginine (15.4 mg, 10.8 umol) in 5 ml of anhydrous dimethylformamide. 2. Add 15.08 ul of TEA to the oligoarginine solution. 3. Add cholesterol chloroformate (..."

2012-08-01T19:52:34Z

Created page with "1. Disolve oligoarginine (15.4 mg, 10.8 umol) in 5 ml of anhydrous dimethylformamide. 2. Add 15.08 ul of TEA to the oligoarginine solution. 3. Add cholesterol chloroformate (..."

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1. Disolve oligoarginine (15.4 mg, 10.8 umol) in 5 ml of anhydrous dimethylformamide.

2. Add 15.08 ul of TEA to the oligoarginine solution.

3. Add cholesterol chloroformate (24.3 mg, 54 umol) in 5 ml of anhydrous tetrahydrofuran slowly to the solution.

4. Stir the solution overnight.

5. Remove solvents by purging dry nitrogen gas through a glass transfer pipette.

6. Rinse off the remaining cholesterol chloroformate and TEA three times with 15 ml of diethyl ether each time.

7. Dialyse for 2 days using MWCO 500 (Spectrum) against deionized distilled water to remove free cholesterol.

8. 14 mg of Chol-R9 can be obtained after freeze drying.

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Synthesis chlo-9Arg - Revision history

Paul.xu: Created page with "1. Disolve oligoarginine (15.4 mg, 10.8 umol) in 5 ml of anhydrous dimethylformamide. 2. Add 15.08 ul of TEA to the oligoarginine solution. 3. Add cholesterol chloroformate (..."