Paul.xu at 02:50, 5 August 2014

2014-08-05T02:50:28Z

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Dissolve 0.86 g of PEI in 10 mL 10% aqueous methanol flask equipped with a condenser at 45 ◦ C under a nitrogen atmosphere. To obtain the PEI-SS with a molar ratio of 1:2 between the reactive group in CBA and the primary amine in PEI respectively, 0.325 g CBA was dissolved in 6 mL methanol respectively and dropwise added to the PEI solution. The reaction mixture was stirred in the dark under nitrogen atmosphere for at least 24 h at 45 ◦ C. Subsequently, 10% excess PEI (0.086 g) in 2 mL methanol was added to consume any unreacted acrylamide groups for another 6 h with stirring. After that the transparent solution was diluted with water to 20 mL and acidified with 10 M HCl to pH 4, and then dialyzed against distilled water (MWCO: 3500) for 2 days to remove the unreacted 800 Da PEI. Lastly, the solution was lyophilized for 2 days to obtain the product

 

Synthesis of biotinylated PEI-SS The biotinylated PEI-SS (BP-SS) was prepared according to the literature. 6 Biotin (30 mg) was activated with NHS (14 mg) and EDC (23 mg) in dimethyl sulfoxide (3 mL). The activated biotin solution was added to PEI-SS (200 mg) in deionized water (12 mL).

The reaction mixture was stirred at 4 ◦ C for 24 h, purified by dialyzing against water for 2 days (MWCO: 3500), lyophilized and defined as BP-SS.

 

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Synthesis of PEI-CBA - Revision history

Paul.xu at 02:50, 5 August 2014