Paul.xu at 13:30, 29 August 2014

2014-08-29T13:30:58Z

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== Synthesis of PHP-s-s-TREN ==

'''DAP + DBP Mix''': 75 mg DAP+ 150 ul MeOH, warm to 50 oC to dissolve, add 366 mg DBP-->350 ul

'''TREN''': 180 mg TREN + 450 ul MeOH-->580 ul

'''Cystamine''': 28 mg + 56 ul water-->73 ul, 1 mg/2.6 ul

 

{| width="361" cellspacing="1" cellpadding="1" border="1"
|-
| 
| DBP 
| DAP 
| TREN 
| Cystamine 
|-
| C1 
| 61 mg 
| 12.5 mg 
| 30 mg 
| 0 
|-
| C2 
| 61 
| 12.5 
| 30 
| 1 mg, 2.6 ul 
|-
| C3 
| 61    
| 12.5
| 30
| 2.5 mg, 6.6 ul 
|-
| C4 
| 61
| 12.5
| 30
| 5 mg, 13.4 ul 
|-
| C5 
| 61
| 12.5
| 30
| 7.5 mg, 20 ul 
|-
| C6 
| 61
| 12.5
| 30
| 10 mg, 26.8 ul 
|}

 

50 oC react o/n

Purify with 1 ml isopropanol, 2X, dissolve 500 ul H2O. Dilute with 10 mM HEPES, pH7.0, 1:10, 1:30, 1:50, 1:100

 

8/25/14

Transfection 293T cells, 1ug DNA /3 ul diluted TR

8/26/14. GFP too weak to see

8/27/14

[[Image:PHP-ss-TREN 8 27 14.JPG]]

8/28/14

[[Image:PHP-ss-TREN 8 28 14.JPG]]

8/29/14

[[Image:PHP-ss-TREN_8_29_14.JPG]]

[[Category:Transfection]] [[Category:Paul]]

Synthesis of PHP-s-s-TREN - Revision history

Paul.xu at 13:30, 29 August 2014