https://eln.excellgen.com/index.php?action=history&feed=atom&title=Synthesis_of_Pullulan-PEI 2026-10-18T02:38:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Synthesis_of_Pullulan-PEI&diff=12445&oldid=prev 2014-07-19T03:25:00Z

<p>Created page with &quot;Dissolve pullulan in DMSO, stire for 0.5 hour. Add 6 mM discuccinmidyl carbonate while stirring Add 6 mM dimethylaminopyridine while stirring React at RT (25 oC) for 5 ho...&quot;</p> <p><b>New page</b></p><div>Dissolve pullulan in DMSO, stire for 0.5 hour.<br /> <br /> Add 6 mM discuccinmidyl carbonate while stirring<br /> <br /> Add 6 mM dimethylaminopyridine&amp;nbsp;while stirring<br /> <br /> React at RT (25 oC) for 5 hour.<br /> <br /> Cool to 4 oC<br /> <br /> add PEI in weight ratios 1:0.25 (PEI is 0.25).<br /> <br /> React o/n (16 hours)<br /> <br /> Precipitate PEI-Pullulan using acetone.<br /> <br /> Wash 5 times with acetone to remove unreacted PEI<br /> <br /> Dry at 37 oC<br /> <br /> <br /> <br /> <br /> <br /> [[Image:Pollulant_PEI.jpg|500px]]&amp;nbsp;<br /> <br /> <br /> <br /> [[Category:Transfection]][[Category:Paul]]</div>

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