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Synthesis of pHP-2 - Revision history
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Paul.xu at 22:31, 26 April 2011
2011-04-26T22:31:51Z
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<p><b>New page</b></p><div>PHP-PEI conjugation optimization <br />
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<br> <br />
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1. Synthesis of PHP <br />
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1.1: 1.5g 1,3-diamino-2-propanol 16.6 mmole <br />
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+ 3 ml MeOH to dissolve <br />
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+ 3.63 g 1,3-dibromo-2-propanol, 16.6 mmole <br />
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1.2: 50 oC for 48 hrs <br />
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1.3: add 5 X5 ml MeOH to wash, spin, remove soluble material, collect pellet. <br />
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1.4: add 5 ml H2O to pellet to dissolve <br />
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1.5: add 30 ml EtOH to precipitate, spin, remove supernatant. <br />
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1.6: dry at 50 oC incubator.---&gt;0.8 g polymer. <br />
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2. Synthesis of PHP-CBA-PEI18 <br />
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<br> <br />
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{| cellspacing="1" cellpadding="1" border="1" style="width: 446px; height: 214px;"<br />
|-<br />
| <br />
| pHP <br />
| CBA <br />
| PEI18<br />
|-<br />
| TR7 <br />
| 50 mg <br />
| 50 mg <br />
| 25 mg<br />
|-<br />
| TR8 <br />
| 50 mg <br />
| 10 mg <br />
| 12.5mg<br />
|-<br />
| TR9 <br />
| 50 mg <br />
| 10 mg <br />
| 25 mg<br />
|-<br />
| TR10 <br />
| 50 mg <br />
| 5 mg <br />
| 12.5 mg<br />
|-<br />
| TR11 <br />
| 50 mg <br />
| 5 mg <br />
| 5 mg<br />
|-<br />
| TR12 <br />
| 50 mg <br />
| 10 mg <br />
| 50 mg<br />
|-<br />
| TR13<br />
| 50 mg<br />
| 10 mg<br />
| 5 mg<br />
|}<br />
<br />
adjust to 1ml with water:MeOH (9:1). <br />
<br />
50 oC incubate o/n. <br />
<br />
the best transfection reagent: TR11<br> <br />
<br />
Make dilution for transfection assay <br />
<br />
[[Category:Transfection]] [[Category:Paul]]</div>
Paul.xu