https://eln.excellgen.com/index.php?action=history&feed=atom&title=TALEN_Construction
TALEN Construction - Revision history
2026-10-18T07:59:43Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=TALEN_Construction&diff=6753&oldid=prev
Paul.xu at 18:51, 27 October 2012
2012-10-27T18:51:12Z
<p></p>
<p><b>New page</b></p><div>https://boglab.plp.iastate.edu/node/add/single-tale <br />
<br />
https://tale-nt.cac.cornell.edu/node/add/talen<br> <br />
<br />
http://talen-hit.cellectis-bioresearch.com/target/talenHitSearch<br> <br />
<br />
http://idtale.kaust.edu.sa/<br> <br />
<br />
http://taleffectors.com/<br> <br />
<br />
http://baolab.bme.gatech.edu/Research/BioinformaticTools/assembleTALSequences.html<br><br />
<br />
'''<u>Destination Vectors Information:</u>''' <br />
<br />
{| cellpadding="5" border="1"<br />
|-<br />
| <u>Assembly Method</u> <br />
| <u>Plasmid Name</u> <br />
| <u>Description</u> <br />
| <u>Addgene</u><br />
|-<br />
| rowspan="12" | Cermak et al. <br />
| pTAL1 <br />
| TALE with natural bacterial activator domain <br />
| [http://www.addgene.org/31031 31031]<br />
|-<br />
| pTAL2 <br />
| TALE with natural bacterial activator domain and no stop <br />
codon <br />
<br />
| [http://www.addgene.org/31033/ 31033]<br />
|-<br />
| pTAL3 <br />
| TALEN with HIS3 marker <br />
| [http://www.addgene.org/31034/ 31034]<br />
|-<br />
| pTAL4 <br />
| TALEN with LEU2 marker <br />
| [http://www.addgene.org/31035/ 31035]<br />
|-<br />
| Sangamo <br />
| Mammalian expression TALEN with 3x FLAG tag and (-152, +63) <br />
backbone <br />
<br />
| Coming Soon<br />
|-<br />
| pTALE64G <br />
| Mammalian expression TALE with 3x FLAG tag, (-152, +63) <br />
backbone, VP64 activation domain, T2A sequence, and eGFP <br />
<br />
| Coming Soon<br />
|-<br />
| pTALE64C <br />
| Mammalian expression TALE with 3x FLAG tag, (-152, +63) <br />
backbone, VP64 activation domain, T2A sequence, and AmCyan <br />
<br />
| Coming Soon<br />
|-<br />
| pCS2TAL3-DD <br />
| 3xFLAG tag, TALEN with obligate heterodimer DD FokI domain <br />
| [http://www.addgene.org/37275/ 37275]<br />
|-<br />
| pCS2TALE3-RR <br />
| TALEN with obligate heterodimer RR FokI domain <br />
| [http://www.addgene.org/37276/ 37276]<br />
|-<br />
| pTAL5-BB <br />
| Yeast expression TAL repressor with PGAL1 Promoter <br />
| [http://www.addgene.org/36033/ 36033]<br />
|-<br />
| pTAL6-BB <br />
| Yeast expression TAL repressor with PTEF1 Promoter <br />
| [http://www.addgene.org/36034/ 36034]<br />
|-<br />
| pCAG-T7-TALEN(Sangamo)-Destination <br />
| Mammalian expression TALEN with Sangamo backbone <br />
| [http://www.addgene.org/37184/ 37184]<br />
|-<br />
| rowspan="1" | Reyon et al. <br />
| JDS70, 71, 74, 78 <br />
| Mammalian expression TALENs with 3x FLAG tag and Sangamo <br />
backbone with different C-terminal RVDs <br />
<br />
| [http://www.addgene.org/talengineering/TALENkit/ TALENkit]<br />
|-<br />
| rowspan="1" | Briggs et al. <br />
| TALEN <br />
| Mammalian expression TALEN <br />
| Unknown<br />
|}<br />
<br />
[[Category:Protocols]] [[Category:GenomeEngineering]]</div>
Paul.xu