https://eln.excellgen.com/index.php?action=history&feed=atom&title=TBS 2026-10-18T03:27:34Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=TBS&diff=3449&oldid=prev 2012-03-04T14:30:45Z

<p>Created page with &quot;== Recipe : 10X TBS == &#039;&#039;&#039;10X TBS&#039;&#039;&#039;: 500 mM Tris.HCl, pH 7.4 and 1500 mM NaCl. 500 ml of 1 M Tris.Cl pH 7.4 &lt;br&gt; NaCl: 88 g ultrapure water to 1 L&lt;br&gt; == 1X TBS: &lt;...&quot;</p> <p><b>New page</b></p><div>== Recipe :&amp;nbsp;10X TBS ==<br /> <br /> &#039;&#039;&#039;10X TBS&#039;&#039;&#039;: 500 mM Tris.HCl, pH 7.4 and 1500 mM NaCl.<br /> <br /> 500 ml of 1 M Tris.Cl pH 7.4 &lt;br&gt;<br /> <br /> NaCl: 88 g <br /> <br /> ultrapure water to 1 L&lt;br&gt;<br /> <br /> <br /> <br /> == 1X TBS: &lt;br&gt; ==<br /> <br /> 50 mM Tris.HCl, pH 7.4 and 150 mM NaCl. &lt;br&gt;<br /> <br /> 100 ml 10X TBS<br /> <br /> 900 ml ddH&lt;sub&gt;2&lt;/sub&gt;O&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> == pH8.0 TBS&lt;br&gt; ==<br /> <br /> 50 ml 1 M Tris-HCl, pH 8.0<br /> <br /> 8.7 g NaCl<br /> <br /> water to 1 L<br /> <br /> <br /> <br /> == 20X TBS ==<br /> <br /> Trizma base, 122 g&lt;br&gt;NaCl, 180 g&lt;br&gt;Distilled water, 1000 ml&lt;br&gt;Mix to dissolve and adjust pH to 8.4 using 6N HCl.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Buffer_Recipe]]</div>

Paul.xu