https://eln.excellgen.com/index.php?action=history&feed=atom&title=TNBS_Assay 2026-10-18T07:44:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=TNBS_Assay&diff=623&oldid=prev 2011-04-10T20:57:06Z

<p>Created page with &quot;TNBS Assay&lt;br&gt;&#039;&#039;&#039;Protocol 1&#039;&#039;&#039; 1. Mix 0.4 ml of DNA-polymer complex (made 20 ug/ml DNA concentration) with 0.4 ml&lt;br&gt;0.1 M Na2B4O7.10H2O. Add 0.02 ml TNBS solution diluted from ...&quot;</p> <p><b>New page</b></p><div>TNBS Assay&lt;br&gt;&#039;&#039;&#039;Protocol 1&#039;&#039;&#039;<br /> <br /> 1. Mix 0.4 ml of DNA-polymer complex (made 20 ug/ml DNA concentration) with 0.4 ml&lt;br&gt;0.1 M Na2B4O7.10H2O. Add 0.02 ml TNBS solution diluted from 1 M stock solution&lt;br&gt;to 15 mg/ml with water&lt;br&gt;2. Incubate for 45 min at room temperature and measure absorbance at 420 nm&lt;br&gt; (absorbance of the solution made from PLL (20,000)-DNA N:P=2 is usually about 0.35.&lt;br&gt;3. As a blank, add 0.02 ml TNBS to 0.8 ml borate buffer<br /> <br /> &#039;&#039;&#039;Protocol &#039;&#039;&#039;2&lt;br&gt;1. Add 20 ÎŒL of sample (in duplicate) to 130 ÎŒL of 0.1 M phosphate buffer (pH = 7.0).&lt;br&gt;2. Add 850 ÎŒL of 1 mM TNBS in borate buffer (pH = 9.0). &lt;br&gt;3. incubate at 37 °C for 1 h.&lt;br&gt;4. Measure OD367.&lt;br&gt;<br /> <br /> [[Category:Protocols]][[Category:Transfection]]</div>

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