Paul.xu at 15:21, 3 May 2018

2018-05-03T15:21:46Z

New page

== HA Tag antibodies ==

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Accession
| Name
| Clone 
| Heavy
| Light
| Epitope
|-
| 1FRG 
| 12CA5 
| FAB 26/9 
|
EVLLVESGGDLVKPGGFLKLSCAASGFTFSSFGMS WVRHTPDKRLEWVATISNGGGYTYYQDSVKGRFT ISRDNAKNTLFLEMTSLKSEDAGLYYCARRERYDE KGFAYWGRGTLVTVSAAKTTAPSVYPLAPVCGDTT GSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTF PAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPA SSTKVDKKIEPR

|
DIVMTQSPSSLTVTAGEKVTMSCKSSQSLFNSG KRKNFLTWYHQKPGQPPKLLIYWASTRESGVPDRF SGSGSGTDFTLT ITSVQAEDLAIYYCQND YSHPLTFGAGTKLELKRADAAPTVSIFPPSSEQL TSGGASVVCFLNNFYPKDINVKWKIDGSERQNGV LNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYT CEATHKTSTSPIVKSFNR

| XDVPDYASLX 
|-
| 1IFH 
| 
| FAB 17/9 
| EVQLVESGGDLVKPGGSLKLSCAASGFSFSSYGM SWVRQTPDKRLEWVATISNGGGYTYYPDSVKGR FTISRDNAKNTLYLQMSSLKSEDSAMYYCARRE RYDENGFAYWGQGTLVTVSAAKTTAPSVYPLAP VCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSL SSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNV AHPASSTKVDKKIEPR
|
DIVMTQSPSSLTVTAGEKVTMSCTSSQSLFNSGKQK NYLTWYQQKPGQPPKVLIYWASTRESGVPDRFTGS GSGTDFTLTISSVQAEDLAVYYCQNDYSNPLTFGGG TKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNN FYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYS MSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN

| XDVPDYAS 
|-
| colspan="6" | <pre>Heavy

1FRG 1 EVLLVESGGDLVKPGGFLKLSCAASGFTFSSFGMSWVRHTPDKRLEWVATISNGGGYTYY 60
EV LVESGGDLVKPGG LKLSCAASGF+FSS+GMSWVR TPDKRLEWVATISNGGGYTYY
1IFH 1 EVQLVESGGDLVKPGGSLKLSCAASGFSFSSYGMSWVRQTPDKRLEWVATISNGGGYTYY 60

1FRG 61 QDSVKGRFTISRDNAKNTLFLEMTSLKSEDAGLYYCARRERYDEKGFAYWGRGTLVTVSA 120
DSVKGRFTISRDNAKNTL+L+M+SLKSED+ +YYCARRERYDE GFAYWG+GTLVTVSA
1IFH 61 PDSVKGRFTISRDNAKNTLYLQMSSLKSEDSAMYYCARRERYDENGFAYWGQGTLVTVSA 120

Light

1FRG 1 DIVMTQSPSSLTVTAGEKVTMSCKSSQSLFNSGKRKNFLTWYHQKPGQPPKLLIYWASTR 60
DIVMTQSPSSLTVTAGEKVTMSC SSQSLFNSGK+KN+LTWY QKPGQPPK+LIYWASTR
1IFH 1 DIVMTQSPSSLTVTAGEKVTMSCTSSQSLFNSGKQKNYLTWYQQKPGQPPKVLIYWASTR 60

1FRG 61 ESGVPDRFSGSGSGTDFTLTITSVQAEDLAIYYCQNDYSHPLTFGAGTKLELKRADAAPT 120
ESGVPDRF+GSGSGTDFTLTI+SVQAEDLA+YYCQNDYS+PLTFG GTKLELKRADAAPT
1IFH 61 ESGVPDRFTGSGSGTDFTLTISSVQAEDLAVYYCQNDYSNPLTFGGGTKLELKRADAAPT 120

</pre>

|}

== Tag Antibodies ==

{| border="1" cellspacing="1" cellpadding="1" style="width: 721px;"
|-
| style="width: 100px;" | Urgency
| style="width: 100px;" | Name
| style="width: 26px;" | Chain
| style="width: 47px;" | Clone ID
| style="width: 171px;" | Name
| style="width: 194px;" | Parent
| style="width: 194px;" | Vector
| style="width: 49px;" | Insert Size
| style="width: 345px;" | Enzymes
| style="width: 345px;" | Status/note
|-
| style="width: 100px;" | 0
| style="width: 100px;" | '''Vector''' 
| style="width: 26px;" | L
| style="width: 47px;" | 3154 
| style="width: 171px;" | pCMV5-HA-VH-mKappa 
| style="width: 194px;" | HA  3129 pMiniT2-3129-1 
| style="width: 194px;" | 2493 pCMV5-mIgG-kLC 
| style="width: 49px;" | 527
| style="width: 345px;" | BamH1-Kpn1 
| style="width: 345px;" | sequenced, good, glycerol stock made
|-
| style="width: 100px;" | <s>3</s>
| style="width: 100px;" | 
| style="width: 26px;" | <s>H</s>
| style="width: 47px;" | <s>3129 </s> 
| style="width: 171px;" | <s>pCMV5-HA-VH-mIgG2b</s> 
| style="width: 194px;" | <s>new 3129 pMiniT with b</s>
| style="width: 194px;" | <s>3154 pCMV5-Flag-H</s> 
| style="width: 49px;" | <s>1034</s> 
| style="width: 345px;" | <s>Kpn1 + Not1</s> 
| style="width: 345px;" | <s>3129-BamH1-F + 3129-Kpn1-F1</s> 
|-
| style="width: 100px;" | -<s>2</s>
| style="width: 100px;" | 
| style="width: 26px;" | <s>L</s>
| style="width: 47px;" | <s>3021</s>
| style="width: 171px;" | <s>pCEL0-mIg-LCKappa</s> 
| style="width: 194px;" | 
| style="width: 194px;" | <s>3016 pCEL0</s> 
| style="width: 49px;" | 
| style="width: 345px;" | <s>Vector 3016 pCEL0:   BssHII  - Nhe1 insert in pMiniT2:  BssHII  - Xba1</s> 
| style="width: 345px;" | <s>PCR from  IgG kappa LC from 2493 pCMV5-mIgG-kLC, 3021-F2  + 3021-Age1-Xba1-R , Vector: BssHII - Nhe1,  insert: BssHII - Xba1, in  pMiniT2 vector now.</s> 
|-
| style="width: 100px;" | -<s>2</s>
| style="width: 100px;" | 
| style="width: 26px;" | <s>H</s>
| style="width: 47px;" | <s>3027 </s> 
| style="width: 171px;" | <s>pCEH0-mIgG2b-HC</s> 
| style="width: 194px;" | <s>3076-pMiniT2</s> 
| style="width: 194px;" | <s>3017 pCEH0</s> 
| style="width: 49px;" | 
| style="width: 345px;" | <s>Nhe1-Not1</s> 
| style="width: 345px;" |
<s>PCR from 2492  pCMV5-mIgG2b-HC  using 3020-Xba1-Nhe1-F + 3027-2b-Not1-Age1-R</s>

<s>in pMiniT2 now, sequenced</s>

|-
| style="width: 100px;" | 0
| style="width: 100px;" | '''His'''
| style="width: 26px;" | H+L, sc
| style="width: 47px;" | 3002 
| style="width: 171px;" | pJet1.2-Anti-His Tag 3D5 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2 
| style="width: 49px;" | 
| style="width: 345px;" | Nde1-Sal1/Xho1
| style="width: 345px;" | 3129-Kpn1-F1, sequenced, good, need to subclone into pET30b, but there is no tag for purification 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | '''His '''
| style="width: 26px;" | L
| style="width: 47px;" | 3001  
| style="width: 171px;" | pMiniT2-His-VL  
| style="width: 194px;" | 
| style="width: 194px;" | 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" |
good, sequenced

|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H 
| style="width: 47px;" | 3135 
| style="width: 171px;" | 3135-3-pMiniT2 
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" |
in pMiniT2 vector now, 3135-3-pMiniT2 sequenced, good,  need to subclone into

3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1

|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | L
| style="width: 47px;" | 3001  
| style="width: 171px;" | pCMV5-His-VL-kappa 
| style="width: 194px;" | 3001-pMiniT2 
| style="width: 194px;" | 3130 pCMV5-HA-VL-mIgKappa 
| style="width: 49px;" | 435
| style="width: 345px;" | BamH1-Kpn1
| style="width: 345px;" |
3001-pMiniT2 is good, sequenced

subclone from 3001-pMiniT2, 

|-
| style="width: 100px;" | -2
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3135
| style="width: 171px;" | pCMV5-His-VH-IgG2b 
| style="width: 194px;" |  3135-3-pMiniT2  
| style="width: 194px;" |
3129 pCMV5-HA-VH-mIgG2b

<s>3009pCMV5-Flag-VH-IgG2b</s>

| style="width: 49px;" | 364
| style="width: 345px;" | BsrG1-Kpn1  
| style="width: 345px;" | subclone into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 from 3135-3-pMiniT2  
|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3168 
| style="width: 171px;" | pCEH0-His-VH-mIgG2a 
| style="width: 194px;" | 3135-3-pMiniT2  
| style="width: 194px;" | 3163 pCEH0-1FRG-HA-VH-mIgG2a 
| style="width: 49px;" | 358
| style="width: 345px;" | BsrG1-Nhe1 
| style="width: 345px;" |
3135-3-pMiniT2 

VHSQVQLQQSGPEDVKPGASVKISCKASGYTFTDYYMNWVKQSPGKGLEWIGDINPNNGGTSYNQKFKGRATLTVDKSSSTAYMELRSLTSEDSSVYYCESQSGAYWGQGTTVTVSAASS

|-
| style="width: 100px;" | 0
| style="width: 100px;" | Myc 
| style="width: 26px;" | L
| style="width: 47px;" | 3003  
| style="width: 171px;" | 3003-L-5 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | IL2 signal peptide, BamH1-Kpn1, good 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3003
| style="width: 171px;" | 3003-H-1 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | need to use 3076-myc-BsrG1-F to fix frame shift
|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | L
| style="width: 47px;" | 3075 
| style="width: 171px;" | pCMV5-Myc-L-kappa 
| style="width: 194px;" | 
| style="width: 194px;" | 2493 pCMV5-mIgG-kLC 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" |
completed, subclone from 3003-L5 using BamH1-Kpn1 to 2493 pCMV5-mIgG-kLC

sequenced, good

|-
| style="width: 100px;" | -2
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3076 
| style="width: 171px;" | pCMV5-Myc-H-IgG2b 
| style="width: 194px;" | 
| style="width: 194px;" |
3129 pCMV5-HA-VH-mIgG2b

| style="width: 49px;" | 
| style="width: 345px;" |  BsrG1-Kpn1  
| style="width: 345px;" |
PCR from 3003-H-1 in pJet1.2, using 3076-myc-BsrG1-F  and  3076-myc-Nhe1-Kpn1-R

in pMiniT2 now sequenced, good

|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3167 
| style="width: 171px;" | pCEH0-Myc-VH-mIgG2a 
| style="width: 194px;" |
3027-pMiniT2

(3076 myc pMiniT2)

| style="width: 194px;" | 3163  pCEH0-1FRG-HA-VH-mIgG2a 
| style="width: 49px;" | 397
| style="width: 345px;" | BsrG1-Nhe1
| style="width: 345px;" |
subcloning from the misslabeled myc clone in pMiniT2

EVDLVESGGDLVKPGGSLKLSCAASGFTFSHYGMSWVRQTPDKRLEWVATIGSRGTYTHYPDSVKGRFTISRDNDKNALYLQMNSLKSEDTAMYYCARRSEFYYYGNTYYYSAMDYWGQGTTVTVSS

|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | sc VH+VL
| style="width: 47px;" | 3074  
| style="width: 171px;" | pT7A30-HA-Avi-BirA 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | 3074-1-PJET1-2 is good, need to subclone into 1834 pT7A30-Avi-BirA 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | L
| style="width: 47px;" | 3011 
| style="width: 171px;" | pJet1.2-HA-L-1 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | 3011-L-1 is good, sequenced 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | H
| style="width: 47px;" | 3011 
| style="width: 171px;" | pJet1.2-HA-H-2 
| style="width: 194px;" | 
| style="width: 194px;" | pJet1.2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" |
3011-H-2 is good, sequenced

|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | L
| style="width: 47px;" | 3030
| style="width: 171px;" | 3130-4-pMiniT2  
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | 3130-4-pMiniT2 is good (sequenced), derived from 3011-L-1  pJet1.2-HA light chain by PCR using 3130-BamH1-R + 3130-Kpn1-R 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | L
| style="width: 47px;" | 3130 
| style="width: 171px;" | pCMV5-HA-VL-mIgKappa 
| style="width: 194px;" |  3130-4-pMiniT2   
| style="width: 194px;" | 2493 pCMV5-mIgG-kLC 
| style="width: 49px;" | 429bp 
| style="width: 345px;" | subclone  from using BamH1+ Kpn1 
| style="width: 345px;" |
completed,good, sequenced, need to make glycerol stock

|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | H
| style="width: 47px;" | 3129
| style="width: 171px;" | pMiniT2-3129-1
| style="width: 194px;" | 3011 pJet1.2-HA Heavy chain 
| style="width: 194px;" | pMiniT2 
| style="width: 49px;" | 
| style="width: 345px;" | PCR
| style="width: 345px;" | good sequenced
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | H
| style="width: 47px;" | 3129 
| style="width: 171px;" | pCMV5-HA-VH-mIgG2b 
| style="width: 194px;" | 2492 pCMV5-mIgG2b-HC
| style="width: 194px;" | 3154 pCMV5-HA-VH-mKappa 
| style="width: 49px;" |  
| style="width: 345px;" | subcloning  Kpn1 -Not1
| style="width: 345px;" |  subcloning mIgG2b constant domain to 3154, due to BamH1 site
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | L
| style="width: 47px;" | 3131  
| style="width: 171px;" | pCEL0-HA-VL-mIgKappa 
| style="width: 194px;" | 3011 pJet1.2-HA-L-1  
| style="width: 194px;" | 3021 pCEL0-mIg-LCKappa 
| style="width: 49px;" | 342 
| style="width: 345px;" | BssHII-Xho1 
| style="width: 345px;" |
subclone 342 bp fragment from 3011 pJet1.2-HA-L-1 using BssHII-Xho1

do not use the smallest fragment

|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1IFH 
| style="width: 26px;" | H
| style="width: 47px;" | 3132  
| style="width: 171px;" | pCEH0-HA-VH-mIgG2a 
| style="width: 194px;" | 3011 pJet1.2-HA-H-2 
| style="width: 194px;" | 3163 pCEH0-1FRG-HA-VH-mIgG2a 
| style="width: 49px;" | 373 
| style="width: 345px;" | BsrG1-Nhe1 
| style="width: 345px;" | subclone 373 bp fragment from 3011 pJet1.2-HA-H-2 using BsrG1-Nhe1
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1FRG 
| style="width: 26px;" | L
| style="width: 47px;" | 3159 
| style="width: 171px;" |  pMiniT2-1FRG-HA-VL-kappa 
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | gene fragment from IDT
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1FRG 
| style="width: 26px;" | H
| style="width: 47px;" | 3160  
| style="width: 171px;" | pMiniT-2-1FRG-HA-VH-mIgG2a 
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | gene fragment from IDT 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1FRG 
| style="width: 26px;" | L
| style="width: 47px;" | 3162 
| style="width: 171px;" |   pCMV5-1FRG-HA-VL-kappa 
| style="width: 194px;" | 
| style="width: 194px;" | 3130 pCMV5-HA-VL-mIgKappa 
| style="width: 49px;" | 767
| style="width: 345px;" | BamH1-Not1
| style="width: 345px;" |  subcloning from 3159
|-
| style="width: 100px;" | <s>1</s>
| style="width: 100px;" | <s>HA, 1FRG</s> 
| style="width: 26px;" | <s>H</s>
| style="width: 47px;" | <s>3158</s> 
| style="width: 171px;" | <s>pCMV5-HA-VH-mIgG2a</s> 
| style="width: 194px;" | 
| style="width: 194px;" | <s>3129 pCMV5-HA-VH-mIgG2b</s> 
| style="width: 49px;" | <s>1471</s>
| style="width: 345px;" | <s>BsrG1-Not1</s>
| style="width: 345px;" | <s>subcloning from 3160</s>
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1FRG 
| style="width: 26px;" | H
| style="width: 47px;" | 3163 
| style="width: 171px;" | pCEH0-1FRG-HA-VH-mIgG2a 
| style="width: 194px;" | 3160 pMiniT-2-1FRG-HA-VH-mIgG2a 
| style="width: 194px;" | 3017 pCEH0 
| style="width: 49px;" | 1464
| style="width: 345px;" | BsrG1-Not1 
| style="width: 345px;" | subcloning from 3160 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | HA, 1FRG 
| style="width: 26px;" | L
| style="width: 47px;" | 3164
| style="width: 171px;" | pCEL0-1FRG-HA-VL-kappa 
| style="width: 194px;" | 3159 pMiniT2-1FRG-HA-VL-kappa 
| style="width: 194px;" |  3016 pCEL0 
| style="width: 49px;" | 701
| style="width: 345px;" | BssHII --NheI 
| style="width: 345px;" | subcloning from 3159 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | Flag 
| style="width: 26px;" | L
| style="width: 47px;" | 3010   
| style="width: 171px;" | 3010-5-miniT2
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | sequenced, good
|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3009 
| style="width: 171px;" | 3009-4-pMiniT2-Flag-H  
| style="width: 194px;" | 
| style="width: 194px;" | pMiniT2 
| style="width: 49px;" | 
| style="width: 345px;" | 
| style="width: 345px;" | 3009-4-pMiniT2, good, sequenced 
|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | L
| style="width: 47px;" | 3010
| style="width: 171px;" | pCMV5-Flag-kappa 
| style="width: 194px;" | 3010-5-miniT2 
| style="width: 194px;" |
<s>2493 pCMV5-Flag-kappa</s>

3130

pCMV5-HA-VL-mIgKappa

| style="width: 49px;" | 
| style="width: 345px;" | BamH1+ Kpn1 
| style="width: 345px;" | need to do subcloning from 3010-5-miniT2
|-
| style="width: 100px;" | -2
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3009
| style="width: 171px;" | pCMV5-Flag-VH-IgG2b 
| style="width: 194px;" | 3009-4-pMiniT2-Flag-H  
| style="width: 194px;" |
 3129 pCMV5-HA-VH-mIgG2b

| style="width: 49px;" |  432
| style="width: 345px;" | BsrG1-Kpn1
| style="width: 345px;" |
subclone from 3009-4-pMiniT2 using BsrG1-Kpn1

|-
| style="width: 100px;" | 0
| style="width: 100px;" | 
| style="width: 26px;" | H
| style="width: 47px;" | 3152 
| style="width: 171px;" | pCEH0-Flag-VH-mIgG2a 
| style="width: 194px;" | 3009-4-pMiniT2  
| style="width: 194px;" | 3163 pCEH0-1FRG-HA-VH-mIgG2a 
| style="width: 49px;" | 371
| style="width: 345px;" |
<s>BsrG1-Nhe1</s>

BsrG1-Kpn1

| style="width: 345px;" |
subclone from 3009-4-pMiniT2 using <s>BsrG1-Nhe1</s>

VHSEVQLQQSGGELAKPGASVKMSCKSSGYTFTAYAIHWAKQAAGAGLEWIGYIAPAAGAAAYNAAFKGKATLAADKSSSTAYMAAAALTSEDSAVYYCARAAAAGADYWGQGTTLTVSSAS

|}

--[[User:Paul.xu|Paul.xu]] ([[User_talk:Paul.xu|talk]]) 07:20, 13 February 2018 (EST)
[[Category:Antibodies]] [[Category:To Do]] [[Category:Tasks]]

Tag Antibodies - Revision history

Paul.xu at 15:21, 3 May 2018