https://eln.excellgen.com/index.php?action=history&feed=atom&title=Tat-Cre_Recombinase_QC 2026-10-19T11:04:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Tat-Cre_Recombinase_QC&diff=14936&oldid=prev 2016-07-01T20:45:58Z

<p></p> <p><b>New page</b></p><div>1. Day1:&amp;nbsp;Transfect 293T&amp;nbsp;cells in 12/24 well plate with<br /> <br /> &lt;span style=&quot;color: rgb(0, 0, 0); font-family: sans-serif; font-size: 12.7px; line-height: 19.05px;&quot;&gt;2181&amp;nbsp;&lt;/span&gt;&amp;nbsp;pCMV-LoxP-kozak-TurboRFP-ODC-2Stops-LoxP-TurboGFP: from Red to Green<br /> <br /> 1522 &amp;nbsp;CMV-Brainbow-2.1-R Plasmid, from Green to Red.<br /> <br /> <br /> <br /> 2. Day2: or 3, Check transfection efficiency with microscope.&amp;nbsp;<br /> <br /> 3. Add Cre recombinase to final conc of&amp;nbsp;2.5 uM,&amp;nbsp;5 uM, 10 uM<br /> <br /> use this page to calculate required amount of Cre Recombinase, MW 43 kDa<br /> <br /> http://www.excellgen.com/pub/mw_2_moles.html<br /> <br /> or&amp;nbsp;<br /> <br /> http://www.molbiol.ru/eng/scripts/01_04.html<br /> <br /> 4. Check color change on day 3, 4, 5, 6, 7, 8&amp;nbsp;under microscope.<br /> [[Category:Protocols]]</div>

Paul.xu