https://eln.excellgen.com/index.php?action=history&feed=atom&title=To-do_This_week To-do This week - Revision history 2026-10-18T04:02:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=To-do_This_week&diff=17882&oldid=prev Paul.xu at 20:48, 8 May 2018 2018-05-08T20:48:53Z <p></p> <p><b>New page</b></p><div>== Item List ==<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 655px;&quot;<br /> |-<br /> | Status<br /> | Clone ID<br /> | style=&quot;width: 60px;&quot; | Name<br /> | style=&quot;width: 360px;&quot; | Desciption<br /> | style=&quot;width: 134px;&quot; | Note<br /> |-<br /> | 1&lt;br/&gt;<br /> | 3102&lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | ABClone3&lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | Sequence with&amp;nbsp;hEF1-HTLV-seq-F (2012_5_15) CCCTTGGAGCCTACCTAG or&amp;nbsp; EF1-HTLV-seq-F (9/14/17) CTGCTTGCTCAACTCTACGTC<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | 1<br /> | 3028&lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | pFuse4&lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | Sequence with SV40pA-seq-R (2012_5_15 A07)&amp;nbsp;&amp;nbsp;GCAATAGCATCACAAATTTC&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | 1<br /> | &lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | look for NTC48165 DH5a dcm- competent cells&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | 1<br /> | &lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | look for&amp;nbsp;192 pCMVS-EGFP plasmid&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | design oligos for 994, 995,&amp;nbsp;988,&amp;nbsp;3179<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | 1<br /> | 3092&lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | make some more DNA for phage display&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | style=&quot;width: 60px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 360px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | &lt;br/&gt;<br /> |}<br /> <br /> == Cloning ==<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | &#039;&#039;&#039;ID&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Status&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Plasmid Name&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Name&#039;&#039;&#039;<br /> | &#039;&#039;&#039;DNA Size&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Vector&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Method&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Status&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Date&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Enzyme&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Expression&#039;&#039;&#039;<br /> | &#039;&#039;&#039;Note&#039;&#039;&#039;<br /> |-<br /> | 3195&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMVS-LAMP-1-BCMA&lt;br/&gt;<br /> | BCMA in LAMP1 vector<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | for&amp;nbsp; genetic immune of BCMA<br /> |-<br /> | 3194&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP&lt;br/&gt;<br /> | BCMA-Fc fusion<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3192&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMV5-PD-1-Avi-6H-BirA&lt;br/&gt;<br /> | PD-1 biotin-6XHis&amp;nbsp;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3191&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pT7A3-GST-Avitag&lt;br/&gt;<br /> | GST-Avitag fusion protein<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | Avi<br /> |-<br /> | 998<br /> | <br /> | pT7A3-6His-NLS-dCas9-NLS-6xHis&lt;br/&gt;<br /> | dCas9<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 1000&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pT7A3-6His-NLS-FnCpf1-NLS-6xHis&lt;br/&gt;<br /> | FnCpf1&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 995&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCold-GST-CD133-EC3&lt;br/&gt;<br /> | CD133-EC3&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 994&amp;nbsp;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCold-GST-CD133-EC2&lt;br/&gt;<br /> | CD133-EC2&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3196&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMV5-CD47-Avi-6H-BirA&lt;br/&gt;<br /> | CD47&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | &lt;span style=&quot;color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;&quot;&gt;889&lt;/span&gt;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMV-PD1-AviTag-c6His&lt;br/&gt;<br /> | PD-1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3197&amp;nbsp;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pT7A3-GST-BCMA&lt;br/&gt;<br /> | GST-BCMA fusion for ELISA screening<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3199&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCMV5-BCMA-Avi-6H-BirA&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 2503&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pCold-VVTopo&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3206&amp;nbsp;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pEF1-HTLV-LAMP-1-BCMA&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:To Do]]</div> Paul.xu