Paul.xu: Created page with "5'-C UGAACGAGAAACGU AAA '''AUGAUAUAA'''AUAUCAA. 3' barl $ ' -CAUC'''AGGA A'''UAUUUGAUUCAG '''AUG AUA UGA '''CUAUCAA- 3' bar ll Me..."

2017-06-09T14:19:48Z

Created page with "5'-C UGAACGAGAAACGU AAA '''AUGAUAUAA'''AUAUCAA. 3' barl $ ' -CAUC'''AGGA A'''UAUUUGAUUCAG '''AUG AUA UGA '''CUAUCAA- 3' bar ll Me..."

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5'-C UGAACGAGAAACGU AAA '''AUGAUAUAA'''AUAUCAA. 3'  barl

$ ' -CAUC'''AGGA A'''UAUUUGAUUCAG '''AUG AUA  UGA '''CUAUCAA- 3'  bar ll

Met Ile stop

barI and barII siles and their nucleotidc sequences in the lambda genome. The double line (top) represents Ihe complete lambda. genome. The first enlargent (middle) shows the genetic context surrounding bari and barII loci The second enlargement (bottom) shows the homologous sequence in barI and BarII, Nucleotides forming part of the open reading frames of  barl and barll are indicated by arrows, b. Sequences of barI barII transcrirrs, Underlined nucleotides are the S-D seqnences and those in boldface indicate the putative ORFs of bar minigenes.

pMiniT

SP6 promoter

CAT TTAGGTGACACTATAGA   AGTGTGTATCGCTCGAGGGATCCGAATTCAGGAGGTAAAAACC '''ATG ATC TGA TAA TAA''' TTA

Minigene: ATG ATC TGA (Met Ile Stop)

[[Category:Cloning]]

Toxic Minigene - Revision history

Paul.xu: Created page with "5'-C UGAACGAGAAACGU AAA '''AUGAUAUAA'''AUAUCAA. 3' barl $ ' -CAUC'''AGGA A'''UAUUUGAUUCAG '''AUG AUA UGA '''CUAUCAA- 3' bar ll Me..."