https://eln.excellgen.com/index.php?action=history&feed=atom&title=Transfection_reagents_2012-1-4
Transfection reagents 2012-1-4 - Revision history
2026-10-18T02:39:00Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Transfection_reagents_2012-1-4&diff=3496&oldid=prev
Paul.xu at 14:45, 9 March 2012
2012-03-09T14:45:40Z
<p></p>
<p><b>New page</b></p><div>guanidinylation <br />
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QC result after 2 months storage at -20 oC: without DSP conjugation the transfection reagent will not work.<br> <br />
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{| cellspacing="1" cellpadding="1" border="1" style="width: 723px; height: 103px;"<br />
|-<br />
| <br />
PHP-ss-DSP-guPEI18, 1:10<br> <br />
<br />
| PHP-ss-DSP-guPEI18, 1:20 <br />
| PHP-ss-DSP-guPEI18, 1:30 <br />
| PHP-ss-DSP-guPEI18, 1:40<br><br />
|-<br />
| PHP-ss-guPEI18, 1:10 <br />
| PHP-ss-guPEI18, 1:20 <br />
| PHP-ss-guPEI18, 1:30 <br />
| PHP-ss-guPEI18, 1:40<br />
|-<br />
| guPEI18, 1:10 <br />
| guPEI18, 1:20 <br />
| guPEI18, 1:30 <br />
| guPEI18, 1:40<br />
|}<br />
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24 hours after transfection <br />
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&nbsp; &nbsp; <br />
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[[Image:2012 1 5 day1 plate1.jpg]]<br> <br />
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[[Image:2012 1 5 day1.jpg]] <br />
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<br> <br />
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<br> <br />
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48 hours after transfection <br />
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[[Image:2012 1 6 day2 plate1.JPG]]<br> <br />
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[[Image:2012 1 6 day2.jpg]] <br />
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<br> <br />
<br />
day3<br> <br> <br />
<br />
[[Image:2012 1 7 day3 plate1.jpg]] <br />
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Day4 <br />
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[[Image:2012 1 8 day4 plate1.JPG]]<br> <br />
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<br> <br />
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day 5 <br />
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[[Image:2012 1 9 day5 plate1.JPG]]<br> <br />
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Plate2<br> <br />
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{| width="600" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| <br />
PHP-ss-DSP-guPEI18, 1:50 <br />
<br />
<br> <br />
<br />
| PHP-ss-DSP-guPEI18, 1:60 <br />
| PHP-ss-DSP-guPEI18, 1:70 <br />
| PHP-ss-DSP-guPEI18, 1:80<br><br />
|-<br />
| PHP-ss-guPEI18, 1:50 <br />
| PHP-ss-guPEI18, 1:60 <br />
| PHP-ss-guPEI18, 1:70 <br />
| PHP-ss-guPEI18, 1:80<br />
|-<br />
| guPEI18, 1:50 <br />
| guPEI18, 1:60 <br />
| guPEI18, 1:70 <br />
| guPEI18, 1:80<br />
|}<br />
<br />
24 hours after transfection <br />
<br />
&nbsp; &nbsp; <br />
<br />
[[Image:2012 1 5 day1 plate2.jpg]] <br />
<br />
<br> <br />
<br />
[[Image:2012 1 5 day1 plate2 2.jpg]] <br />
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<br> <br />
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48 hours after transfection <br />
<br />
[[Image:2012 1 6 day2 plate2.JPG]] <br />
<br />
[[Image:2012 1 6 day2 plate2 1.jpg]] <br />
<br />
day3 <br />
<br />
[[Image:2012 1 7 day3 plate2.jpg]] <br />
<br />
day4<br> <br />
<br />
[[Image:2012 1 8 day4 plate2.JPG]] <br />
<br />
<br> <br />
<br />
Day5 <br />
<br />
[[Image:2012 1 9 day5 plate2.JPG]] <br />
<br />
<br> <br />
<br />
2011-1-10: Do transfection using more diluted polymer <br />
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{| cellspacing="1" cellpadding="1" border="1" style="width: 723px; height: 103px;"<br />
|-<br />
| <br />
PHP-ss-DSP-guPEI18, 1:80<br> <br />
<br />
| PHP-ss-DSP-guPEI18, 1:100 <br />
| PHP-ss-DSP-guPEI18, 1:120 <br />
| PHP-ss-DSP-guPEI18, 1:150<br><br />
|-<br />
| PHP-ss-guPEI18, 1:80 <br />
| PHP-ss-guPEI18, 1:200 <br />
| PHP-ss-guPEI18, 1:120 <br />
| PHP-ss-guPEI18, 1:150<br />
|-<br />
| control, 11-10-2011 <br />
| control, 11-10-2011 <br />
| <br />
| <br />
|}<br />
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[[Image:2012 1 11.jpg]]<br> <br />
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[[Category:Transfection]]</div>
Paul.xu