https://eln.excellgen.com/index.php?action=history&feed=atom&title=Transfection_reagents_2012-1-9 Transfection reagents 2012-1-9 - Revision history 2026-10-18T07:28:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Transfection_reagents_2012-1-9&diff=8462&oldid=prev Paul.xu at 03:53, 6 March 2013 2013-03-06T03:53:55Z <p></p> <p><b>New page</b></p><div>Polymer made on 1/6/2012&lt;br&gt; <br /> <br /> Modifier PEI18+Gu: 0.5 g 1H-Pyrazole-1-carboxamidine hydrochloride, 1 g PEI18, 11 ml water&lt;br&gt; <br /> <br /> Modifier Gu: 1H-Pyrazole-1-carboxamidine hydrochloride, 100 mg/ml in water.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 371px; height: 360px;&quot;<br /> |-<br /> | Code&lt;br&gt; <br /> | width=&quot;120&quot; | Reaction&lt;br&gt; <br /> | Volume of Polymer&lt;br&gt; <br /> | Volume of Modifier&lt;br&gt; <br /> | Water&lt;br&gt;<br /> |-<br /> | 1PP&lt;br&gt; <br /> | width=&quot;120&quot; | PEI18+ Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 50 uL&lt;br&gt; <br /> | 200 uL&lt;br&gt;<br /> |-<br /> | 2PP&lt;br&gt; <br /> | width=&quot;120&quot; | PEI18+ Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 100 uL&lt;br&gt; <br /> | 150 uL&lt;br&gt;<br /> |-<br /> | 3PP&lt;br&gt; <br /> | width=&quot;120&quot; | PEI18+ Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 150 uL&lt;br&gt; <br /> | 100 uL&lt;br&gt;<br /> |-<br /> | 4PP&lt;br&gt; <br /> | width=&quot;120&quot; | PEI18+ Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 200uL&lt;br&gt; <br /> | 50 uL&lt;br&gt;<br /> |-<br /> | 5PP&lt;br&gt; <br /> | width=&quot;120&quot; | PEI18+ Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 250 uL&lt;br&gt; <br /> | 0 uL&lt;br&gt;<br /> |-<br /> | 1P&lt;br&gt; <br /> | width=&quot;120&quot; | Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 50 uL&lt;br&gt; <br /> | 200 uL&lt;br&gt;<br /> |-<br /> | 2P&lt;br&gt; <br /> | width=&quot;120&quot; | Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 100 uL&lt;br&gt; <br /> | 150 uL&lt;br&gt;<br /> |-<br /> | 3P&lt;br&gt; <br /> | width=&quot;120&quot; | Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 150uL&lt;br&gt; <br /> | 100uL&lt;br&gt;<br /> |-<br /> | 4P&lt;br&gt; <br /> | width=&quot;120&quot; | Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 200uL&lt;br&gt; <br /> | 50uL&lt;br&gt;<br /> |-<br /> | 5P&lt;br&gt; <br /> | width=&quot;120&quot; | Gu&lt;br&gt; <br /> | 1 ml&lt;br&gt; <br /> | 250uL&lt;br&gt; <br /> | 0uL&lt;br&gt;<br /> |-<br /> | Control&lt;br&gt; <br /> | width=&quot;120&quot; | -&lt;br&gt; <br /> | 1 m&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> Reaction condition: RT o/n&lt;br&gt; <br /> <br /> 24 hours after transfection &lt;br&gt; <br /> <br /> plate1&lt;br&gt; <br /> <br /> [[Image:24h plate1 1.10.2012.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> plate2&lt;br&gt; <br /> <br /> [[Image:24h plate2 1.10.2012.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> plate3&lt;br&gt; <br /> <br /> [[Image:24h plate3 1.10.2012.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> plate4&lt;br&gt; <br /> <br /> [[Image:24h plate4 1.10.2012.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 48 hours after transfection&lt;br&gt; <br /> <br /> plate1&lt;br&gt; <br /> <br /> [[Image:2012 1 11 plate1.JPG]]&lt;br&gt; <br /> <br /> plate2 <br /> <br /> [[Image:2012 1 11 plate2.JPG]]&lt;br&gt; <br /> <br /> plate3 <br /> <br /> [[Image:2012 1 11 plate3.JPG]]&lt;br&gt; <br /> <br /> plate4&lt;br&gt; <br /> <br /> [[Image:2012 1 11 plate4.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> day 3 <br /> <br /> plate1 <br /> <br /> [[Image:Day3 plate1 1.12.2012s.JPG]] <br /> <br /> plate2 <br /> <br /> [[Image:Day3 plate2 1.12.2012s.JPG]] <br /> <br /> plate3 <br /> <br /> [[Image:Day3 plate3 1.12.2012s.JPG]] <br /> <br /> plate4 <br /> <br /> [[Image:Day3 plate4 1.12.2012s.JPG]] <br /> <br /> day 4 <br /> <br /> plate1 &lt;br&gt; <br /> <br /> [[Image:Day4 plate1 1.13.2012.JPG]] <br /> <br /> plate2&lt;br&gt; <br /> <br /> [[Image:Day4 plate2 1.13.2012.JPG]] <br /> <br /> plate3&lt;br&gt; <br /> <br /> [[Image:Day4 plate3 1.13.2012.JPG]] <br /> <br /> plate4 <br /> <br /> [[Image:Day4 plate4 1.13.2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Transfection]]</div> Paul.xu