<?xml version="1.0"?>
<feed xmlns="http://www.w3.org/2005/Atom" xml:lang="en">
	<id>https://eln.excellgen.com/index.php?action=history&amp;feed=atom&amp;title=Transfection_reagents_comparison_13</id>
	<title>Transfection reagents comparison 13 - Revision history</title>
	<link rel="self" type="application/atom+xml" href="https://eln.excellgen.com/index.php?action=history&amp;feed=atom&amp;title=Transfection_reagents_comparison_13"/>
	<link rel="alternate" type="text/html" href="https://eln.excellgen.com/index.php?title=Transfection_reagents_comparison_13&amp;action=history"/>
	<updated>2026-10-18T03:21:02Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
	<generator>MediaWiki 1.44.0</generator>
	<entry>
		<id>https://eln.excellgen.com/index.php?title=Transfection_reagents_comparison_13&amp;diff=2130&amp;oldid=prev</id>
		<title>Luyingying at 14:43, 29 October 2011</title>
		<link rel="alternate" type="text/html" href="https://eln.excellgen.com/index.php?title=Transfection_reagents_comparison_13&amp;diff=2130&amp;oldid=prev"/>
		<updated>2011-10-29T14:43:44Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
Make new transfection reagent (TR). &amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
3 ul TR + 50ul DMEM without serum----&amp;amp;gt;Mix A&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
1 ug DNA + 50ul DMEM without serum----&amp;amp;gt;Mix B&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
Mix A + Mix B, incubate 20min at room temperature &lt;br /&gt;
&lt;br /&gt;
Add 100 ul mixture to transfect 293T cells in 12 well-plate.&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[[Image:GeneExpressoMax QC 10 26 2011.jpg]]&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;amp;nbsp;48 hours after transfection, picture taken on 10-26-2011 &amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
&amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
[[Image:GeneExpressoMax QC blue 10 26 2011.JPG|541x378px|GeneExpressoMax QC blue 10 26 2011.JPG]]&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
48 hours after transfection, picture taken on 10-26-2011 &amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
[[Image:GeneExpressoMax QC 72hblue 10 28 2011.JPG]]&amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
72 hours after transfection, picture taken on 10-28-2011 &amp;lt;br&amp;gt; &lt;br /&gt;
&lt;br /&gt;
[[Image:Transfection_QC_10_29_2011.jpg]] &lt;br /&gt;
&lt;br /&gt;
10-29-2011 &amp;lt;br&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
[[Category:Transfection]]&lt;/div&gt;</summary>
		<author><name>Luyingying</name></author>
	</entry>
</feed>