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Transfection reagents comparison 14 - Revision history
2026-10-18T03:26:31Z
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Luyingying at 14:54, 28 November 2011
2011-11-28T14:54:58Z
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<p><b>New page</b></p><div>*'''Make 7 different transfection reagents (TR).'''<br />
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3 ul TR + 50ul DMEM without serum----&gt;Mix A <br />
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1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum----&gt;Mix B <br />
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Mix A + Mix B, incubate 20min at room temperature <br />
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Add 100 ul mixture to transfect 293T cells in 12 well-plate.<br> <br />
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1-1,1-2,2-1,2-2,3-1 are EG-1031, lot 20111107. <br />
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4-1,4-2 are lot 20110930. <br />
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[[Image:SEVEN TR 11.4.2011.JPG|502x384px|SEVEN TR 11.4.2011.JPG]] <br />
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48 hours after transfection, picture taken on 11-4-2011<br> <br />
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[[Image:SEVEN TR 11.7.2011.JPG]]<br> <br />
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48 hours after transfection, picture was taken on 11-7-2011.<br> <br />
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*'''Make 4 different transfection reagents (TR). '''<br />
<br />
3 ul TR + 50ul DMEM without serum----&gt;Mix A <br />
<br />
1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum----&gt;Mix B <br />
<br />
Mix A + Mix B, incubate 20min at room temperature <br />
<br />
Add 100 ul mixture to transfect 293T cells in 12 well-plate. <br />
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1 is EG-1086, lot 20110714. <br />
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2 is EG-1074, lot 20110803 (Gene Expresso 8000). <br />
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3 is EG-1031, lot 20111107. <br />
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4 is EG-1086T, lot 20111110. <br> <br />
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[[Image:11.11.2011.jpg]]<br> <br />
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48 hours after transfection, picture was taken on 11-11-2011.<br> <br />
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[[Category:Transfection]]</div>
Luyingying