Paul.xu at 14:55, 8 February 2011

2011-02-08T14:55:22Z

New page

Notebook: Zhen Tao #100

Page: 001913

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[[Image:Transfection reagent comparison 2 one day after transfection2.JPG]]

1 day after transfection<br> --[[User:Paul.xu|Paul.xu]] 14:14, 5 February 2011 (EST) <br>

[[Image:Transfection reagent comparison 2 two days after transfection2.JPG]]

2 days after transfection

--[[User:Paul.xu|Paul.xu]] 10:33, 6 February 2011 (EST)

[[Image:Transfection reagent comparison 2 three days after transfection.JPG]]

3 days after transfection

--[[User:Paul.xu|Paul.xu]] 09:09, 7 February 2011 (EST)

[[Image:Transfection reagent comparison 2 four days after transfection.JPG]]

4 days after transfection

--[[User:Paul.xu|Paul.xu]] 09:52, 8 February 2011 (EST)

{| cellspacing="1" cellpadding="1" border="1" style="width: 588px; height: 324px;"
|-
| well number
| transfection reagent
| amount of TR being used (ul)
| DNA(PSMPUW-CMV-EGFP) (ug)
|-
| 1
| LPEI25
| 1
| 1
|-
| 2
| LPEI25
| 3
| 1
|-
| 3
| BPEI25
| 1
| 1
|-
| 4
| BPEI25
| 3
| 1
|-
| 5
| PEIMAX
| 1
| 1
|-
| 6
| PEIMAX
| 3
| 1
|-
| 7
| PolyExpress
| 3
| 1
|-
| 8
| GeneExpresso
| 3
| 1
|-
| 9
| GeneExpresso Plus
| 3
| 1
|-
| 10
| Proteinone
| 2
| 1
|-
| 11
| Proteinone
| 4
| 1
|-
| 12
| TurboFect
| 3
| 1
|}

1) Add transfection reagent to 50 ul DMEM

2) Add DNA to 50 ul DMEM

3) Add transfection reagent in step 1) to DNA (step 2), mix well and keep for 20 min at room temperature

4) Add the mixture in step 3 to cells in 12 well plate.

--[[User:Ztao|Ztao]] 15:03, 4 February 2011 (EST)

[[Category:Transfection]]

Transfection reagents comparison 2 - Revision history

Paul.xu at 14:55, 8 February 2011