Paul.xu at 17:00, 19 February 2011

2011-02-19T17:00:36Z

New page

Notebook: Zhen Tao #100

Page: 

 

{| cellspacing="1" cellpadding="1" border="1" style="width: 588px; height: 324px;"
|-
| well number
| transfection reagent
| amount of TR being used (ÎŒl)
| DNA(PSMPUW-CMV-EGFP) (ÎŒg)
|-
| 1
| LPEI25
| 1
| 1
|-
| 2
| LPEI25
| 3
| 1
|-
| 3
| BPEI25
| 1
| 1
|-
| 4
| BPEI25
| 3
| 1
|-
| 5
| PEIMAX
| 1
| 1
|-
| 6
| PEIMAX
| 3
| 1
|-
| 7
| PolyExpress
| 3
| 1
|-
| 8
| GeneExpresso
| 3
| 1
|-
| 9
| GeneExpresso Plus
| 3
| 1
|-
| 10
| Proteinone
| 6
| 1
|-
| 11
| TurboFect
| 1
| 1
|-
| 12
| TurboFect
| 3
| 1
|}

1) Add transfection reagent to 50 ÎŒl DMEM (no serum!) 

2) Add DNA to 50 ÎŒl DMEM

3) Add transfection reagent in step 1) to DNA (step 2), mix well and keep for 20 min at room temperature

4) Add the mixture in step 3 to cells in 12 well plate.

 

[[Image:Transfection reagent comparison 3 1 day after transfection.JPG]]

1 day after transfection --[[User:Ztao|Ztao]] 13:33, 8 February 2011 (EST)

[[Image:Transfection reagent comparison 3 2 day after transfection.JPG]]

day 2 after transfection --[[User:Ztao|Ztao]] 11:45, 9 February 2011 (EST)

 

[[Image:Transfection reagent comparison 3 3 day after transfection.JPG]]

3 day after transfection --[[User:Ztao|Ztao]] 09:17, 10 February 2011 (EST)

 

[[Image:Transfection reagent comparison 3 12 day after transfection.JPG]]

12 days after transfection

--[[User:Paul.xu|Paul.xu]] 12:00, 19 February 2011 (EST)

[[Category:Transfection]]

Transfection reagents comparison 3 - Revision history

Paul.xu at 17:00, 19 February 2011