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Transfection reagents freeze-thaw 100 times - Revision history
2026-10-18T05:18:54Z
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Luyingying at 16:21, 26 November 2011
2011-11-26T16:21:10Z
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<p><b>New page</b></p><div>Make transfection reagent (TR) to freeze-thaw 100 times. <br />
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3 ul TR + 50ul DMEM without serum----&gt;Mix A <br />
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1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum----&gt;Mix B <br />
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Mix A + Mix B, incubate 20min at room temperature <br />
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Add 100 ul mixture to transfect 293T cells in 12 well-plate. <br> <br />
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0 is control. <br />
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48 hours after transfection<br> <br />
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[[Image:Plate3s 11.23.2011.jpg]]<br> <br />
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[[Image:Plate3s 11.23.2011.JPG]]<br> <br />
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2011-11-24 (3 days after transfection) <br />
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[[Image:2011 11 24 freeze thaw stability.jpg]]<br> <br />
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[[Image:2011_11_26_freeze_thaw_stability.jpg]]<br />
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--[[User:Luyingying|Xiaojing Wang]] 12:04, 23 November 2011 (EST)<br> <br />
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[[Category:Transfection]]</div>
Luyingying