Luyingying: Created page with "Make transfection reagent (TR) to freeze-thaw 10 times. 3 ul TR + 50ul DMEM without serum---->Mix A 1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B Mix A + Mix B..."

2011-11-22T20:31:47Z

Created page with "Make transfection reagent (TR) to freeze-thaw 10 times. 3 ul TR + 50ul DMEM without serum---->Mix A 1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B Mix A + Mix B..."

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Make transfection reagent (TR) to freeze-thaw 10 times.

3 ul TR + 50ul DMEM without serum---->Mix A

1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B

Mix A + Mix B, incubate 20min at room temperature

Add 100 ul mixture to transfect 293T cells in 12 well-plate.

[[Image:TR FREEZE 0-10 11.4.2011.JPG]]

48 hours after transfection, picture taken on 11-4-2011

[[Image:TR FREEZE 0-10 11.7.2011.JPG]]

Picture was taken on 11-7-2011.

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[[Category:Transfection]]

Transfection reagents freeze-thaw 10 times - Revision history

Luyingying: Created page with "Make transfection reagent (TR) to freeze-thaw 10 times. 3 ul TR + 50ul DMEM without serum---->Mix A 1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum---->Mix B Mix A + Mix B..."