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Transfection reagents freeze-thaw each 2 weeks - Revision history
2026-10-18T08:19:36Z
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Paul.xu at 20:53, 21 February 2012
2012-02-21T20:53:18Z
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<p><b>New page</b></p><div>Make transfection reagent (TR,lot 20111107) to freeze-thaw each 2 weeks. lot 20111107<br> <br />
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3 ul TR + 50ul DMEM without serum----&gt;Mix A <br />
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1 ug DNA(pCMV-EGFP) + 50ul DMEM without serum----&gt;Mix B <br />
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Mix A + Mix B, incubate 20min at room temperature <br />
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Add 100 ul mixture to transfect 293T cells in 12 well-plate. <br />
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0 is control. (2011.11.7)<br> <br />
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2 is TR frozen 2 weeks. (2011.11.21)<br> <br />
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48 hours after transfection <br />
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[[Image:Freeze-thaw 2 weeks s.jpg]]<br> <br />
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[[Image:Freeze-thaw 2 weeks blue s.jpg]]<br> <br />
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--[[User:Luyingying|Xiaojing Wang]] 16:16, 23 November 2011 (EST)<br> <br />
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0 is control. (2011.11.7) <br />
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4 is TR frozen 4 weeks. (2011.12.5) <br />
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48 hours after transfection <br> <br />
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[[Image:TR FREEZE 2 weeks 12.8.2011.JPG]] <br />
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[[Image:TR FREEZE 2 weeks blue 12.8.2011.JPG]]<br> <br />
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--[[User:Yingying.lu|Xiaojing Wang]] 13:52, 8 December 2011 (EST) <br />
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0 is control. (2011.11.7) <br />
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6 is TR frozen 6 weeks. (2011.12.5) <br />
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48 hours after transfection <br> <br />
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[[Image:2 weeks 12.19.2011.jpg]]<br> <br />
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[[Image:2 weeks blue 12.19.2011.jpg]]<br> <br />
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0 is control. (2011.11.7) <br />
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9 is TR frozen 9 weeks. (2012.1.9) <br />
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24 hours after transfection <br> <br />
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[[Image:24h 2 weeks 1.10.2012.jpg]]<br> <br />
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[[Image:Day1 2 weeks 1.10.2012.jpg]] <br />
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day 2 after transfection<br> <br />
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[[Image:Day2 2 weeks 1.11.2012.jpg]]<br> <br />
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day 3 after transfection <br> <br />
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[[Image:Day3 2 weeks 1.12.2012.jpg]]<br> <br />
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0 is control. (2011.11.7) <br />
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12 is TR frozen 12 weeks. (2012.2.1) <br />
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48 hours after transfection <br> <br />
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[[Image:12weeks.JPG]]<br> <br />
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[[Image:12weeks blue.JPG]]<br> <br />
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0 is control. (2011.11.7) <br />
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15 is TR frozen 15 weeks. (2012.2.17) <br />
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4 days after transfection <br> <br />
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[[Image:15 weeks.jpg]]<br> <br />
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[[Image:15 weeks blue.jpg]]<br />
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[[Category:Transfection]]</div>
Paul.xu